Quantification of anandamide,oleoylethanolamide and palmitoylethanolamide in rodent brain tissue using high performance liquid chromatographyelectrospray mass spectroscopy  

作　　者：Daniel J.Liput Eleftheria Tsakalozou Dana C.Hammell Kalpana S.Paudel Kimberly Nixon Audra L.Stinchcomb 

机构地区：[1]Department of Pharmaceutical Sciences,College of Pharmacy,University of Kentucky,Lexington,KY 40536,USA [2]AllTranz,Lexington,KY 40505,USA [3]Department of Pharmaceutical Sciences,College of Pharmacy,South College,Knoxville,TN 37922,USA [4]Department of Pharmaceutical Sciences,School of Pharmacy,University of Maryland,Baltimore,MD 21201,USA

出　　处：《Journal of Pharmaceutical Analysis》2014年第4期234-241,共8页药物分析学报（英文版）

基　　金：supported by funding from the National Institute ofAlcohol Abuse and Alcoholism AA016959(KN),AA016499(KN)and AA019853(DJL)and the Kentucky Science andTechnology Corporation(KSTC-184-512-07-029;ALS)

摘　　要：Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEAReported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEA

关 键 词：Endocarmabinoids Acylethanolamides ANANDAMIDE OEA PEA LC-MS 

分 类 号：Q95-3[生物学—动物学]