益生菌VSL#3对大鼠实验性结肠炎大肠组织TLR4及NF-κBp65表达的影响  

Effects of VSL#3 on TLR4 and NF-κBp65 expression of experimental colitis in rats

作　　者：桑力轩[1] 高楠[1] 张岱[1] 陈玉帅[1] 刘姝[1] 温博[1] 王炳元[1] 崔勇[2] 姜敏[3] 

机构地区：[1]中国医科大学附属第一医院老年消化科,辽宁沈阳110001 [2]辽宁中医药大学解剖组胚教研室 [3]中国医科大学附属第一医院消化科

出　　处：《胃肠病学和肝病学杂志》2014年第8期964-966,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：沈阳市科学技术计划项目(f12-277-1-54)

摘　　要：目的探讨大鼠实验性结肠炎TLR4/NF-κBp65信号转导通路及VSL#3的干预作用。方法 5%葡聚糖硫酸钠(DSS)溶液自由饮用1周构建大鼠急性实验性结肠炎模型,观察和评价大肠黏膜的组织学损伤,计算DAI评分及大鼠病理学评分。应用Real-time PCR方法检测大肠组织TLR4及NF-κBp65 mRNA的表达;Western blotting方法检测大肠组织TLR4及NF-κBp65表达水平。结果益生菌VSL#3组大鼠一般情况明显好于模型组。DAI评分:益生菌VSL#3组DAI评分较模型组低(P<0.05),益生菌VSL#3组病理学评分较模型组低(P<0.05);Real-time PCR及Western blotting检测TLR4及NF-κBp65的表达水平:与模型组相比,益生菌VSL#3组TLR4表达水平明显下降(P<0.05);模型组NF-κBp65的表达明显高于益生菌VSL#3组及正常对照组(P<0.05)。结论益生菌VSL#3通过调节TLR4/NF-κBp65信号转导通路减轻大鼠实验性结肠炎。Objective To investigate the effect of VSL#3 on TLR4 and NF-κBp65 expression of experimental colitis in rats. Methods 5% dextran sulfate sodium （DSS） solution free drank a week to build a model of acute experimental colitis rat, colon mucosa damage tissue, pathological DAI score and the score of rats were observed and evaluated. Real- time PCR method was used to detect the expression of TLR4 method was used to detect TLR4 and NF-κBp65 expression and NF-κBp65 mRNA in colorectal tissue; Western blotting of colorectal tissues. Results The rats of probiotic VSL#3 group was significantly better than the model group. DAI score ： the DAI score and pathology score of probiotics VSL#3 group were lower than those of the model group （P 〈 0.05 ）. Real-time PCR and Western blotting detection of TLR4 and NF-κBp65： compared with the model group, the expression of TLR4 in probiotics VSL#3 group was significantly de- creased （P 〈 0.05 ） ; the expression of NF-κBp65 in model group was significantly higher in the probiotic VSL#3 group and the normal control group （P 〈0.05）. Conclusion Probiotic VSL#3 attenuates experimental colitis in rat by regu- lating the TLR4/NF-κBp65 signaling pathway.

关 键 词：炎症性肠病 TOLL样受体 核因子-ΚBP65 

分 类 号：R574.1[医药卫生—消化系统]