核桃楸皮化学成分的鉴定及其抗肝癌活性成分筛选  

Chemical compositions of Cortex Juglans Mandshurica and their activity screening of anti-hepatocarcinoma effect

作　　者：曹治家 蔡小燕[1] 方薇[1] 陈洪升[1] 张怀念[1] 张咏莉[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《山东医药》2014年第29期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金青年科学基金项目(81102753);中山市华南现代中药城(健康医药产业基地)专项发展资金项目(2009H021)

摘　　要：目的鉴定核桃楸皮的化学成分,并对其进行体外抗肝癌活性成分筛选。方法采用75%乙醇回流法提取核桃楸皮,经分级萃取、硅胶柱层析及制备型高效液相色谱分析等方法得到单体化合物,通过化合物的理化性质及核磁共振技术鉴定化合物成分;采用MTT法评价其体外抗肝癌活性。结果从核桃楸皮中分离得到2种黄酮类化合物,经鉴定分别为木犀草素和刺芒柄花素。MTT检测结果表明,木犀草素对肝癌细胞HepG2和正常肝细胞LO2都有一定的毒性作用,IC50分别为(103.32±21.34)、(131.13±13.48)μmol/L;刺芒柄花素对肝癌细胞HepG2的毒性作用较小,其IC50>200μmol/L。结论核桃楸皮含2种黄酮类化合物:木犀草素和刺芒柄花素,其中木犀草素具有明显的抗肝癌作用。Objective To investigate the chemical compositions of Cortex Juglans Mandshurica and to evaluate anti-tumor effects of these compounds in human hepatoma cells.Methods Cortex Juglans Mandshurica were extracted with 75%ethanol, concentrated with chloroform and ethyl acetate, eluted radiantly on silica gel, and purified by preparative HPLC.Physicochemi-cal constants and spectroscopic analysis were used to identify their structures .MTT assay was used to evaluate their anti-hepato-carcinoma bioactivities.Results Two compounds were isolated and elucidated as luteolin and formononetin.Luteolin had strong cytotoxic effect on HepG2 and LO2 with IC50 values of （103.32 ±21.34） and （131.13 ±13.48） μmol/L;Formononetin showed lighter cytotoxic effect on HepG2 with IC50〉200μmol/L.Conclusion Luteolin and formononetin are isolated from Cortex Jug-lans Mandshurica for the first time, and luteolin shows significant anti-tumor activity in human hepatoma cells.

关 键 词：肝肿瘤 核桃楸皮 化学成分 

分 类 号：R735.7[医药卫生—肿瘤] R282.5[医药卫生—临床医学]