基于核磁共振氢谱代谢组学研究黄连解毒汤对胰岛素抵抗大鼠棕色脂肪组织代谢组的影响  

Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics

作　　者：杨永霞[1] 王琳琳[1,2] 郑凌云 王淑美 黄榕波[1] 张磊[1] 黄耀庭[1] 

机构地区：[1]广东药学院基础学院 [2]中药学院,广州510006

出　　处：《高等学校化学学报》2014年第9期1883-1888,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21005022)资助~~

摘　　要：采用基于核磁共振氢谱(1H NMR)的代谢组学方法,研究了黄连解毒汤(HJD)对高果糖诱导胰岛素抵抗大鼠模型棕色脂肪代谢组的影响.选取Wistar大鼠32只,适应7 d后随机分为正常对照组、模型组、阳性药物对照组和黄连解毒汤组,每组8只.正常对照组给予纯净水喂养,其它3组给予100 g/L的果糖水喂饲.28 d后,4组大鼠除了继续给予100 g/L的果糖水喂养外,阳性对照组和黄连解毒汤组同时分别给予阿托伐他汀10 mg/(kg·d)和HJD水煎剂3.175 g/(kg·d)灌胃,正常对照组和模型组给予一定体积的生理盐水灌胃,整个实验持续56 d.取各组大鼠棕色脂肪组织(BAT),采集各组组织提取液的1H NMR谱,运用主成分分析法(PCA)分析.与正常对照组相比,在模型组中乳酸、胆碱、磷脂胆碱/甘油磷脂胆碱、肌酸/肌酸酐、牛磺酸和肌苷的含量升高,脂质含量降低;黄连解毒汤组逆转了模型组中上述各代谢物的变化,且引起肌醇升高,均具有统计学意义.实验结果表明,黄连解毒汤能够逆转机体能量代谢、减轻细胞膜受损以及降低肝肾损伤,初步阐明了黄连解毒汤对胰岛素抵抗状态下棕色脂肪组织代谢的调控作用.With the application of 1H nuclear magnetic resonance（1 H NMR） spectroscopy, we studied the effect of Huanglian Jiedu decoction（ HJD） on the metabonomics of brown adipose tissue extracts in high fruc-tose-induced insulin resistance model. 32 Wistar rats were divided into four groups, i. e. , controls, model group, positive drug group and HJD treated group, 8 in each group. The last three groups were given 100 g/L fructose water for 28 d to establish insulin resistance model, normal control group was given the equal volume of pure water at the same time. After 28 d, four groups of rats were given 100 g/L fructose water continuously. Rats in positive drug group were administered orally atorvastain at a dose of 10 mg/（ kg·d） , and rats in HJD treated group were given by gavage with HJD water. The control and model groups were given by gavage with a certain volume of saline solution, and the experiment lasted 56 d. Brown adipose tissues were obtained and 1 H NMR spectra of each sample was performed and analyzed by principal component analysis（ PCA） method. Compared with the control group, lactate, alanine, choline, phosphocholine/glycerol phosphocholine （ PC/GPC） , creatine/creatinine, taurine and inosine increased in the model and lipid decreased. In comparison with model group, the myo-inositol was increased in HJD group, and HJD had reversed the metabolites in the model group. HJD can regulate the body’ s energy metabolism and reduce the damaged cell membrances and the injury of liver and kidney. Therefore, this study elucidates the metabolic mechanism of HJD on insulin re-sistance

关 键 词：核磁共振氢谱 代谢组学 黄连解毒汤 胰岛素抵抗 

分 类 号：O657.2[理学—分析化学]