结缔组织铺片脂肪组织油红染色在组织学实验教学中的应用  

Staining method of adipose tissue in the stretched preparation of loose connective tissue for the experimental teaching of medical students

作　　者：王俊梅[1] 平苏宁 汪泓[1] 黄锦桃[1] 陈大堤[1] 李朝红[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室,广州510080

出　　处：《中国组织化学与细胞化学杂志》2014年第4期382-384,共3页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(81070124);广东省自然科学基金资助(S2012010009199);中华医学会医学教育分会2010年度医学教育研究项目资助(20100203);2013年广东省教学改革项目资助(50000-3211412);中山大学本科教学改革项目研究资助(2011:序号46;2013:50000-1163015;50000-1163018)

摘　　要：目的在传统结缔组织铺片基础上开展脂肪组织油红染色方法在医学本科生组织学实验教学中的应用。方法学生先进行疏松结缔组织铺片,并施行脂肪组织油红o-甲苯胺兰-伊红三重染色,然后镜下观察。结果油红o染色把结缔组织中的脂肪细胞内脂滴保存下来并染上红色。脂肪组织中央的细胞脂滴均匀红染,充满胞浆,周边的脂肪细胞胞浆中油红染色很少,细胞呈空泡状,显示出脂肪细胞亚群存在。甲苯胺兰染色使得疏松结缔组织中肥大细胞染成紫红色,胞核染色浅,细胞数量多、成群分布。伊红可使得结缔组织内除脂肪细胞、肥大细胞意外的其他细胞的胞浆和胶原纤维染成淡红色。结论传统的组织学平铺片技术基础上引入脂肪油红o-甲苯胺兰-伊红三重染色,可增强学生动手能力,并能很好地了解输送结缔组织中细胞的不同表型和分布,丰富组织学内容,把教学、科研连接一起,达到提高实验教学质量的目的。Objective To establish a method to detect adipose tissue in the connective tissue for ex-perimental teaching of histology and embryology of medical undergraduates based on the traditional con-nective tissue spread .Methods After loose connective tissue spread adipose tissue was stained with oil red-toluidine blue-eosin triple staining ,and observed by the optic microscopy .Results The fat droplets in the adipose cells of the connective tissue with oil red staining represented red color .The adipose cells located in the center of adipose tissue evenly filled with red lipid droplets ,while the adipose cells located in the pe-riphery of adipose tissue demonstrated no fat droplets ,indicating different phenotypes of fat cells existed in adipose tissue .Toluidine blue staining demonstrated the clustered mast cells in the loose connective tis-sue in blue-colored cytoplasma ,and light nuclei .The other cells and collagen fibers in the connective tis-sue stained by eosin represented pink .Conclusion The oil red-toluidine blue-eosin triply staining can clearly represent more cell types ,e .g .mast cells ,and adipose cells in the loose connective tissue than other stai-ning methods .Students performed the experiment by themselves can easily master the more useful experi-mental skills and can better understand the different phenotype and the distribution of cells in the loose connective tissue .This might reduce the gap between basic medical teaching and scientific research and a-chieve the goal of improving the quality of experimental teaching .

关 键 词：组织学实验教学 疏松结缔组织平铺片 脂肪组织 油红 o-甲苯胺兰-伊红三重染色 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]