丙酮/(NH4)_(2)SO_(4)双水相体系提取芦丁影响因素研究  

Study on the Influence Factors of Rutin Extraction by Acetone-ammonium Sulfate Aqueous Two-phase System

作　　者：罗亚东[1,2] 胡吉卓 杨银月 韦万兴[1] 

机构地区：[1]广西大学化学化工学院，广西南宁　530004 [2]南宁市一锋生物科技有限公司，广西南宁 530004

出　　处：《广西科学院学报》2014年第3期185-189,共5页Journal of Guangxi Academy of Sciences

基　　金：广西自然科学基金项目(2011GXNSFD018016);广西高校大学生创新创业计划项目(1301053)资助

摘　　要：【目的】研究丙酮/(NH4)2SO4双水相萃取体系对分离槐米中芦丁的影响因素。【方法】利用正交设计法进行优化,并把28.57%的硫酸铵溶液与等体积的70%的丙酮混合溶液,调至pH=9形成的双水相,两相的体积比(V上/V下)是1.62。【结果】萃取体系的上相中芦丁含量达到95.92%。【结论】丙酮/(NH4)2SO4双水相萃取体系分离芦丁具有良好的选择性。Objective]Study on the influence factors of Rutin Extraction from Flos Sophorae Immaturus by acetone?ammonium sulfate aqueous two?phase system.[Methods]The tech-nique conditions was optimized by orthogond test.The aqueous two-phase system (pH 9.0) consisted of aqueous (NH4)2SO4solution (28.57%,W%)and acetone (70%,W%)in equal volume,and ratio of volume of two phases (Vl/Vh ,Vl is light phase,Vh is heavy phase)is 1.62.[Results]By this extract system,the content of rutin in light phase is 95.92%.[Conclu-sion]This result revealed that the aqueous liquid two?phase system was effective for separa-ting rutin.

关 键 词：槐米 芦丁 双水相 

分 类 号：TQ460.6[化学工程—制药化工]