绿原酸对缺氧复氧心肌细胞内ankyrin-B的调节作用及其机制  

Effect and mechanism of chlorogenic acid on ankyrin-B of cardiomyocytes under hypoxia reoxygenation

作　　者：贺文凤[1,2] 申阳[1] 刘欣[1] 曹青[1,2] 洪葵[1,2] 

机构地区：[1]南昌大学第二附属医院心内科,南昌330006 [2]江西省分子医学重点实验室

出　　处：《临床心血管病杂志》2014年第8期725-726,共2页Journal of Clinical Cardiology

基　　金：教育部创新团队基金(No:IRT1141);教育部博士点基金(No:20113601110002)

摘　　要：目的:探讨绿原酸对缺氧复氧导致的心肌细胞内锚定蛋白-B(ankyrin-B)下降的调节作用及其机制。方法:培养乳鼠心肌细胞,分为对照组、缺氧复氧组和绿原酸处理组。以流式细胞法检测心肌细胞内活性氧(ROS)的水平,用Western-blot检测心肌细胞内ankyrin-B的表达。结果:缺氧复氧后心肌细胞内ROS水平明显增高,绿原酸预处理能够抑制ROS水平的增高[对照组:(237.6±22.3);缺氧复氧组:(612.1±25.6),绿原酸处理组:(375.9±30.1);P<0.05]。缺氧复氧后心肌细胞内ankyrin-B的表达显著降低,绿原酸预处理能够上调ankyrin-B的表达。结论:绿原酸可能通过清除细胞内的ROS从而减少缺氧复氧引起的心肌细胞内ankyrin-B的表达降低。Objective： To investigate the effect and the mechanism of chlorogenic acid on the reduction of ankyrin-B induced by hypoxia/reoxygenation in cardiomyocytes. Method： Cultured cardiomyocytes of neonatal SD rats had been divided into control, hypoxia/reoxygenation （H/R） and hypoxia/reoxygenation ＋ Chlorogenic acid （H/R＋CGA） group. The level of reactive oxygen species（ROS）was detected by flow cytometry and the expres- sion of ankyrin-B in cardiomyocytes was detected by western-blot. Result：The level of ROS was significantly in- creased by H/R and the increasing was decreased by pretreatment with chlorogenic acid [control group.. （237.6±22.3）, H/R group： （612.1±25.6）,H/R＋CGA group： （375.92=30.1） ;P〈0.05]. The expression of ankyrin-B in cardiomyocytes was significiantly decreased by H/R and was increased by pretreatment with chlorogenic acid. Conclusion： Chlorogenic acid was able to prevent cardiomyocytes injury induced by H/R by regulating ankyrin-B.

关 键 词：锚定蛋白-B 绿原酸 活性氧 

分 类 号：R542.2[医药卫生—心血管疾病]