养血清脑颗粒对大鼠脑缺血再灌注损伤后神经干细胞增殖分化的影响  

The effects of Yangxue Qingnao Granule on proliferation and differentiation of neural stem cells in MCAO model rats

作　　者：楼小亮[1] 席秋江[1] 胡雪勇[1] 李晓萍[1] 范华[1] 谭建萍[1] 

机构地区：[1]南昌大学第四附属医院神经内科南昌,330003

出　　处：《中国神经精神疾病杂志》2014年第7期394-399,共6页Chinese Journal of Nervous and Mental Diseases

基　　金：江西省卫生厅中医药科研重点项目(编号:2008Z010)

摘　　要：目的探讨养血清脑颗粒对内源性神经干细胞增殖分化的影响和神经保护机制。方法应用TTC染色和免疫荧光双标技术观察脑缺血再灌注损伤后3 d、7 d、14 d、21 d神经缺损程度、脑梗死体积、Brdu平均荧光强度值和Brdu+Nestin荧光强度测定及治疗后的影响。结果 1除假手术组外,模型对照组、养血清脑低剂量组和养血清脑高剂量大鼠均出现不同程度的神经功能缺损和脑梗死,各组大鼠不同时间点神经缺损评分和脑梗死体积均不一样,差异均有统计学意义(P<0.01);2治疗后第3、7、14、21天侧脑室下区(subventricular zone,SVZ)和海马齿状回颗粒下层(subgranular zone,SGZ)Brdu阳性细胞或Nestin阳性细胞其平均荧光强度值不同时间点间比较均有统计学差异(P<0.01)。低、高剂量组Brdu阳性细胞平均荧光强度值和Brdu+Nestin免疫双标荧光强度值均高于模型组(P<0.01)。结论养血清脑颗粒能促进缺血再灌注脑损伤大鼠神经功能恢复减轻脑梗死体积,对脑缺血再灌注损伤后神经干细胞增殖分化有促进作用。Objective To investigate the effects of Yangxueqingnao Granule on the proliferation and differentiation of endogenous neural stem cells and their neuroprotective mechanism after brain ischemia. Methods Cerebral ischemia was induced in rats by temporary middle cerebral artery occlusion. Ischemic animals received Yangxue Qingnao Granule at high and low doses at days 3,7, 14,and 21 after MCAO. Neurological score and TTC staining were used to assess the neurological deficits and the volume of cerebral infarction, respectively. Immunohistochemistry was used to detect Brdu and Nestin expression in the subventricular zone（SVZ）and hippocampal dentate gyrus subgranular zone（SGZ）region. Results The rats in MCAO control group, low dose group and high dose group showed varying degrees of neurological def-icits and the volume of cerebral infarctions. The neurological defect scores and volume of cerebral infarction were signifi-cantly different at different time points in each group （ P〈0.01）. 2. The mean fluorescence intensity values of Brdu posi-tive cells or the Nestin positive cells in SVZ and SGZ regions were significantly different （ P〈 0.01） at different time points after treatment for 3,7,14 or 21 days. The mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ regions were significant different among low dose group, high dose group and MCAO con-trol group. The mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ re-gions were significantly different among low dose group, High dose group and control group. High dose group significantly increased the mean fluorescence intensity values of Brdu positive cells or the Nestin positive cells in SVZ and SGZ re-gions. Conculsions Yangxue Qingnao Granule is able to promote the functional recovery and reduce the volume of cere-bral infarction in ischemic rats. Yangxue Qingnao Granule can enhance the proliferation and differentiation of endoge-nous neural stem cell

关 键 词：养血清脑颗粒 MCAO模型大鼠 神经干细胞 

分 类 号：R743.3[医药卫生—神经病学与精神病学]