NS-398抑制SKOV3细胞恶性生物学特性的机制  

The mechanism of inhibition of NS-398 on malignant biological characteristics of ovarian cancer

作　　者：赵婷婷[1] 凌斌[1] 冯定庆[2] 申震[1] 周颖[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科,合肥230001 [2]安徽医科大学附属省立医院分子医学重点实验室,合肥230001

出　　处：《安徽医科大学学报》2014年第9期1210-1213,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81072127;81372779;81372777);安徽省自然科学基金(编号:11040606M176;11040606M178)

摘　　要：目的探讨环氧合酶2(COX2)抑制剂NS-398对卵巢癌细胞恶性生物学特性的影响。方法以卵巢癌细胞株SKOV3为研究对象,根据NS-398的处理分为对照组、实验组。利用MTS法和免疫组化法检测Ki-67蛋白表达,从而监测NS-398对SKOV3细胞增殖的影响;采用划痕实验和侵袭实验分别观察细胞迁移和侵袭能力的变化,采用RT-PCR法检测基质金属蛋白酶(MMP)mRNA的表达情况;采用实时荧光定量PCR及Western blot检测上皮间质转化(EMT)相关分子表达情况。结果 1 MTS增殖实验表明NS-398对SKOV3细胞增殖作用无影响,免疫组化结果与之一致;2划痕实验和侵袭实验分别显示,NS-398显著下调SKOV3细胞的迁移和侵袭能力,差异有统计学意义(P<0.05)。RT-PCR检测结果表明NS-398抑制MMP的mRNA表达;3 Western blot结果表明NS-398上调SKOV3细胞E-cadherin的表达,下调Vimentin、Slug、Snail的表达。结论 COX2抑制剂通过阻止MMP的表达和EMT的发生,从而下调SKOV3细胞的迁移、侵袭能力。Objective To investigate effects of cyclooxygenase-2 （COX2） inhibitor NS-398 on the biological char- acteristics of malignant ovarian cancer. Methods This study had ovarian cancer cell line SKOV3 as the research object. According to NS-398 treatment, this study was divided into control group, the experimental group. The use of MTS method and immunohistochemical expression of Ki-67 predicted the effect of NS-398 on proliferation and growth of SKOV3 cell, and scratch experiments and invasion assay to respectively detect the changes of migration and invasiveness capability and analysis of RT-PCR in matrix metalloproteinase （MMP） mRNA, and Western blot to detect the expression of epithelial-mesenchymal transition （EMT） related molecule. Results①MTS prolifera- tion assay suggested that NS-398 had no influence on proliferation of SKOV3 cell （P 〉 0. 05 ） , and consistent with the results of immunohistochemistry.② Scratch experiments and invasion assay respectively showed NS-398 signifi- cantly clown-regulate invasiveness and migration of SKOV3 cells, the differences were statistically significant （P 〈 0. 05）. RT-PCR test results showed that NS-398 inhibited the mRNA expression of MMP.③ In Western blot, it was found that NS-398 could increase E-cadherin expression and reduce Vimentin, slug, snail expression in ovarian cancer cells. Conclusion NS-398 inhibits migration and invasion of SKOV3 cell by clown-regulating expression of MMP and preventing the occurrence of EMT.

关 键 词：卵巢上皮癌 NS-398 迁移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]