红花加速正畸牙齿移动相关机制的初步研究  

The pilot study of the mechanism of Safflower in the acceleration of tooth movement

作　　者：徐建光[1] 沈军[1] 杨梓[1] 谢晋[1] 祝小鹏[1] 

机构地区：[1]安徽医科大学口腔医学院、安徽医科大学附属口腔医院、安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2014年第9期1333-1335,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽医科大学校基金(编号:2012XKJ021)

摘　　要：选取48只8周龄雌性SPF级Wister大鼠,建立大鼠正畸牙移动实验动物模型,随机分为红花组和对照组,红花组每日灌服6 g/kg红花水煎剂,对照组每日灌服3 ml生理盐水,每周加力一次,4周后处死所有实验大鼠,分离大鼠上、下颌骨,测量上颌第一磨牙近中移动距离,并制作第一磨牙及相关牙周组织切片,采用免疫组织化学方法检测牙周组织中血管内皮生长因子(VEGF)的表达,并采用计算机图像分析方法对各组VEGF的表达强度进行半定量分析,同时测量下颌第一磨牙根尖区的骨密度,对所得实验数据进行统计学分析。红花组第一磨牙近中移动距离以及破骨细胞数大于对照组,差异有统计学意义(P<0.05);VEGF在红花组大鼠牙周组织中的表达明显高于对照组(P<0.05);两组下颌第一磨牙区的骨密度差异无统计学意义(P>0.05)。Abstract Forty-eight 8-week-old SPF female Wister rats were chosen in the experiment. They were divided into two groups-Safflower group and control group randomly. In the Safflower group, each rat was drenched with Safflow- er decoction every day （6 g/kg） ; and in the control group, each rat was given 3 ml NS every day. The force of or- thodontic appliances was enhanced every week. All rats were executed 4 weeks later and jaws were separated. The distances of the tooth movement were measured and slices from the periodontal tissue of the maxillary first molars were observed under the optical microscope. Immunohistochemical method was used to examine the expression of VEGF in the periodontal tissue. At the same time, the bone density of the apical area of the first molars was meas- ured. Data were analyzed using method of statistics. The distances of the tooth movement in the Safflower group were greater than the control group. The number of osteoclasters in the Safflower group was more than the control group （P 〈 0.05 ）. The expression of VEGF in the group of Safflower was significantly different compared with the control group （ P 〈 0.05 ） ; there was no significant difference in the bone density （ P 〉 O. 05 ）.

关 键 词：红花 牙移动 WISTER大鼠 

分 类 号：R783.5[医药卫生—口腔医学]