肺癌转移相关microRNA miR-29b的生物信息学分析  

Bioinformatics analysis of lung cancer metastasis-related miR-29b

作　　者：王红艳[1] 郑少秋[1] 涂永生[2] 张雅洁[1] 

机构地区：[1]广州医科大学病理教研室,广州市510182 [2]广州医科大学生理教研室,广州市510182

出　　处：《中国肿瘤临床》2014年第16期1021-1025,共5页Chinese Journal of Clinical Oncology

基　　金：教育部博士点基金博导类课题(编号:20134423110001);广东省自然科学基金项目(编号:S2012010010181);广州市科技计划项目(编号:2014Y2-00171);广州市教育系统创新学术团队项目(编号:13C06);广东省医学科研基金项目(编号:A2013247);广州市属高校科研项目(编号:2012C135)资助~~

摘　　要：目的:应用生物信息学分析A549细胞中在CD133阳性低表达的miR-29b的靶基因及其功能,为以miR-29b为靶点的肿瘤研究提供线索。方法:利用miRNA PCR芯片筛选A549细胞中CD133阳性和CD133阴性差异表达的miRNA,选用miRecords预测miR-29b的靶基因,合并已证实的靶基因,利用GOEAST和DAVID数据库对所得靶基因进行功能富集分析和信号转导通路富集分析。结果:A549细胞中与CD133阴性比较,miR-29b在CD133阳性中表达下调。miR-29b靶基因有106个,其靶基因功能富集于结合和细胞外基质形成等作用(P<0.01);信号转导通路显著富集于JAK-STAT和TGF-β等信号转导通路(P<0.05)。结论:miR-29b可能与肺癌转移相关,miR-29b的靶基因显著富集在与肿瘤相关的信号通路中。Objective：This paper aims to bioinformatically analyze the target genes of miR-29b and to provide clues for cancer research targeting miR-29b. Methods：The differential expression levels of miRNAs in CD133＋and CD133-A549 cells were detected using the miRNA PCR chip. Real-time polymerase chain reaction was performed to verify the partially differential expression of miR-NAs. Target genes of miR-29b were predicted by miRecords and analyzed by gene ontology and signal transduction pathway enrich-ment analysis. Results： The miR-29b expression was significantly decreased in the CD133＋A549 cells compared with that in the CD133-cells. The number of miR-29b target genes was 106. The functions of these target genes were enriched in binding and extracel-lular matrix structural constituent （P〈0.01）. The JAK-STAT and TGF-βsignal transduction pathways were significantly enriched （P〈0.05）. Conclusion：The abnormal expression of miR-29b may be related to metastasis. Some of the predicted target genes of miR-29b were significantly enriched in the signaling pathways in relation to the tumors.

关 键 词：miR-29b 肺癌 靶基因 生物信息学 

分 类 号：R734.2[医药卫生—肿瘤]