五指山小型猪腹腔粘连模型的建立  

Establishment of Peritoneal Adhesion Model in Wuzhishan Minipigs

作　　者：潘金春[1,2] 闵凡贵[1,2] 王希龙[1,2] 陈瑞爱[3] 王凤国[3] 罗书明[3] 

机构地区：[1]广东省实验动物监测所,广州510663 [2]广东省实验动物重点实验室,广州510663 [3]广东大华农动物保健品股份有限公司,新兴527400

出　　处：《实验动物与比较医学》2014年第4期299-302,共4页Laboratory Animal and Comparative Medicine

基　　金：广东省科技计划项目（20118020305002,20118060400020,20128060300001）;广州市科技计划项目（11A66100095）;广东省教育部产学研结合项目（20128091100466）

摘　　要：目的 建立五指山小型猪的腹腔粘连模型.方法 五指山小型猪术前禁食不禁水过夜,手术前用速眠新Ⅱ、戊巴比妥钠联合麻醉,然后采用浆膜物理损伤、化学损伤、血管结扎、腹膜壁层结扎等多因素方法建立五指山小型猪腹腔粘连模型.结果 术后1d即可观察到五指山小型猪形成了腹腔粘连,粘连程度评分可达2分,粘连面积评分也达到2～3分;术后3d观察发现形成较为严重的粘连,粘连程度和面积评分达到3～4分;腹腔粘连维持时间较长,术后12个月仍可见粘连,粘连程度和面积评分达到2～3分.性别间比较,腹腔粘连的程度及面积差别并不明显.结论 采用多因素方法成功建立了五指山小型猪腹腔粘连模型.Objective To establish a peritoneal adhesion model in Wuzhishan minipigs. Methods Fourteen Wuzhishan minipigs were anesthetized by Sumianxin II with intramuscular injec- tion and pentobarbital sodium with intravenous injection after fasting overnight. Then, the abdominal cavity were opened under laparotomy. Afterwards, the peritoneal adhesion model was established by physical damage of serosal, chemical damage, vascular ligation and ligation of parietal peritoneum. Result Peritoneal adhesions were found 1 day after the operation with the degree of adhesion score 2 and adhesion area score 2-3. Peritoneal adhesions were serious 3 days after the operation with the degree of adhesion and adhesion area score 3 -4. The adhesions was sustained for a long time, and peritoneal adhesions still existed 12 months after the operation with the degree of adhesion and adhesion area score 2-3. There were no obvious difference in the degree of adhesion and adhesion area between males and females. Conclusion The peritoneal adhesion model were succeesfully established in Wuzhishan minipigs by multifactors.

关 键 词：五指山小型猪 腹腔粘连模型 多因素 

分 类 号：R-332[医药卫生]