G-CSFR IV型异构体在成年急性髓系白血病的表达及其临床意义  

Expression of G-CSFR IV Isoform in Adult Acute Myeloid Leukemia and Its Clinical Significance

作　　者：吴勇[1] 陈莹莹[1] 陈元仲[1] 

机构地区：[1]福建省血液病研究所、福建医科大学附属协和医院血液科,福建福州350001

出　　处：《中国实验血液学杂志》2014年第4期899-902,共4页Journal of Experimental Hematology

基　　金：国家重点专科建设项目;福建省血液学重点实验室经费(2009J1004);福建医科大学科技重大建设项目经费(09ZD001)

摘　　要：本研究旨在探讨G-CSFR IV型异构体在成年急性白血病患者中的表达及其临床意义。以19例正常骨髓造血干细胞为对照,利用定量RT-PCR检测正常对照、99例成年AML和34例ALL患者的G-CSFR IV/G-CSFR I型异构体的相对表达水平,并对其中84例AML(非M3)患者的临床特征及化疗效果进行分析。结果表明,GCSFR IV/G-CSFR I相对表达水平在AML患者较ALL患者及正常造血干细胞组高,而G-CSFR IV/G-CSFR I的相对表达水平在ALL患者组与正常造血干细胞组差别无统计学意义。AML(非M3)患者的临床特征及疗效分析显示,G-CSFR IV/G-CSFR I相对高表达的患者比低表达的患者的临床CR率低。G-CSFR IV/G-CSFR I相对表达水平与AML(非M3)患者的性别、年龄、幼稚细胞比例、FAB分型、染色体和融合基因所作的危险分层之间无相关性。结论:G-CSFR IV异构体异常高表达与急性髓系白血病预后差相关。This study was aimed to investigate the expression of granulocyte colony-stimulating factor receptor IV （ G- CSFR IV） in adult acute leukemia patients and its clinical significance. The bone marrow hematopoietic stem cells from healthy persons were used as controls. The real-time RT-PCR was used to determine the expression level of G-CSFR I - IV in 99 AML, 34 ALL patients and 19 healthy persons. The results showed that the relative expression level of G-CSFR IV/G-CSFR I in AML patients was obviously elevated, as compared with that in ALL patients and controls, while the relative expression level of G-CSFR IV/G-CSFR I in ALL patients showed no statistical difference from controls. The analysis of clinical features and chemotherapentic efficacy demonstrated that the clinical remission rate in patients with high expression of G-CSFR IV/G-CSFR I was lower than that in patients with low expression. The relative expression level of G-CSFR IV/G-CSFR I was not related with risk stratification from sex, age, blast ratio, FAB typing, chromosome and fusion gene. It is concluded that the abnormal high expression of G-CSFR IV relates with poor prognosis of AML.

关 键 词：G-CSFRⅣ异构体 急性髓系白血病 急性淋细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤]