南瓜蛋白抑制骨髓瘤细胞系RPMI8226 Notch信号表达  

Inhibitory Effect of Pumpkin Protein on Expression of Notch Signal in RPMI8226 Myeloma Cells

作　　者：刘庭波[1] 杨沛 谢捷明[3] 胡建达[1] 

机构地区：[1]福建省血液病研究所,福建省血液病学重点实验室,福建医科大学附属协和医院 [2]福建省医学科学研究院,福建福州350001 [3]福建医科大学药学院,福建福州350001

出　　处：《中国实验血液学杂志》2014年第4期1012-1015,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(307725587);福建省血液病学重点实验室(编号:2009j1004);福建省高校新世纪优秀人才计划(编号NCETFJ-0705);福建省自然科学基金(C0510012;2011J1188)

摘　　要：本研究探讨南瓜蛋白(cucurmosin,CUS)在体外对骨髓瘤细胞RPMI8226增殖抑制的分子机制。用Western blot法检测不同浓度CUS作用骨髓瘤细胞后Notch信号通路Notch1、Jagged-2、P-Akt和NF-kB的表达水平,结果显示,CUS可以下调骨髓瘤细胞中Notch1、Jagged-2和NF-kB蛋白的表达并呈时间和浓度依赖性,同时降低BCL-2和P-Akt的表达。结论:CUS对体外培养的RPMI8226细胞具有明显的增殖抑制作用,并且能明显下调Notch信号及其下游靶基因的表达水平,因此Notch信号通路可能作为多发性骨髓瘤治疗的新靶点,而CUS也可能成为潜在的调控Notch信号通路的新药之一。This study was aimed to explore the inhibitory effect of pumpkin protein （ cucurmosin, CUS） on prolife- ration of RPMI8226 myeloma cells in vitro and its mechanism. Western blot was used to detect the expression level of Notch-l, Jagged-2, P-Akt and NF-KB in the myeloma cells treated by different concentrations of CUS. The results demonstrated that CUS could down-regulate the protein expression levels of Notchl, Jagged-2, P-Akt and NF-KB in the myeloma cells and with time-and concentration-dependent way, at the same time CUS could also decrease the expressions of BCL-2 and P-Akt. It is concluded that CUS can obviously inhibit the RPMI8226 cell proliferation in vitro, down-regulate the expression levels of Notch signal and its down-stream target genes. Therefore, Notch signaling pathway can be used as a new treatment target for multiple myeloma, and CUS may be become a potential new drug for regulating Notch signaling pathway.

关 键 词：南瓜蛋白 骨髓瘤细胞RPMI8226 Notch信号通路 

分 类 号：R733.3[医药卫生—肿瘤]