二烯丙基二硫对人小细胞肺癌NCI-H446细胞裸鼠移植瘤生长的影响  

Effects of DADS on the xenograft growth of NCI-H446 human small cell lung cancer cells in nude mice

作　　者：刘德勇 张庆丽 赵其辉 彭和人 谢海龙[2] 

机构地区：[1]湖南环境职业技术学院病理教研室,衡阳医学硕士421005 [2]南华大学肿瘤研究所,衡阳421001

出　　处：《医学研究生学报》2014年第8期802-805,共4页Journal of Medical Postgraduates

基　　金：湖南省教育厅优秀青年基金(04B057)

摘　　要：目的二烯丙基二硫(diallyl disulfide,DADS)在多种肿瘤治疗研究中取得了显著的成果。文中探讨DADS对人小细胞肺癌细胞裸鼠移植瘤生长的影响,并研究其作用机制。方法取裸鼠25只,建立NCI-H446细胞裸鼠移植瘤模型。成瘤裸鼠按随机列表法分为阳性对照组、阴性对照组、DADS 20mg/kg组、DADS 60mg/kg组、DADS 180mg/kg组,顺铂(DDP)浓度66mg/kg为阳性对照组,等渗盐水作阴性对照。定期观察浓度不同的DADS对肿瘤生长的影响;HE染色,利用光学显微镜观察移植瘤的形态特征;通过流式细胞术实验,验证DADS对瘤组织细胞周期分布及凋亡的影响。结果 DADS 20mg/kg组、60mg/kg组、180mg/kg组抑瘤率(40.6%、53.1%、66.4%)较阴性对照组明显升高(0),差异有统计学意义(P<0.05);与DADS 20 mg/kg组比较,DADS 60 mg/kg组、DADS 180 mg/kg组抑瘤率差异有统计学意义(P<0.05);HE染色后,阴性对照组瘤细胞密度大,瘤细胞排列紊乱,细胞核异型性大,染色深,核分裂像增多,可见明显的细胞坏死。而DADS处理组,细胞密度较小,瘤细胞排列较规则,核异型性小,呈圆形淡染,核分裂像减少。流式细胞术检测显示,DADS 180 mg/kg组、DADS 60mg/kg组处于G2/M期的细胞数(33.1个、24.8个)较阴性对照组(8.5个)明显增多,差异有统计学意义(P<0.05)。结论 DADS能抑制人小细胞肺癌裸鼠移植瘤生长,并对细胞周期产生阻滞,促进细胞凋亡。Objective Diallyl disulfide （ DADS） has achieved remarkable effects in treatment and research of diverserfied cancers.The article was to explore the effects and the mechanism of DADS on the xenograft growth of human small cell lung cancer （ SCLC） cells in nude mice . Methods A total of 25 nude mice were selected to establish xenograft model of NCI-H446 human SCLC cells.The nude mice bearing with SCLC H446 were divided into 5 groups by random selection：positive control group（DDP 66 mg/kg）, negative control group（physiological saline）, 20 mg/kg DADS group, 60 mg/kg DADS group and 180 mg/kg DADS group, which is 40.6%, 53.1%and 66.4%, respectively.The growth of xenograft tumor in mice was observed after being treated with differ-ent concentrations of DADS .The morphological changes of the tumors were examined under light microscopy .Phase distribution and apoptosis of xenograft cells were analyzed by flow cytometry （ FCM） . Results The growth of xenograft tumor were inhibited signifi-cantly by DADS, resulting in decreased cell density and cellular atypia .Moreover, xenograft cell cycle was blocked in G 2/M and cell apoptosis rate was enhanced . Conclusion DADS can significantly inhibit the growth of NCI-H446 cells and lead to apoptosis .

关 键 词：二烯丙基二硫 小细胞肺癌 裸鼠移植瘤 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]