不同抗原修复方法对内翻性乳头状瘤石蜡切片Pan染色的影响  

Different methods of antigen retrieval impact on Pan of sinonasal inverted papilloma

作　　者：于博[1] 李晨[2] 王辉[1] 葛莹[1] 孔慧[1] 孔力[2] 

机构地区：[1]大连医科大学附属第二医院耳鼻咽喉科,辽宁大连116027 [2]大连医科大学组胚实验室

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第17期1311-1313,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金项目(No:31371218)

摘　　要：目的:探讨不同修复条件及修复液对细胞角蛋白(广谱)单克隆抗体(Pan)的阳性表达程度的影响,筛选优势抗原修复方法。方法:对11例鼻腔鼻窦内翻性乳头状瘤(SNIP)组织石蜡切片,分别采用高温高压EDTA修复、微波EDTA修复、微波柠檬酸钠修复、高温高压柠檬酸钠修复,利用Image-Pro Plus图像分析仪对每张切片随机取5个视野,平均计算200个单位染色区域面积,测定染色区阳性反应的平均吸收度值。结果:高温高压柠檬酸钠修复、微波EDTA修复、微波柠檬酸钠修复与高温高压EDTA修复Pan的阳性表达分别为0.324±0.051、0.325±0.056、0.303±0.061及0.365±0.023;前3种方法与高温高压EDTA修复比较,均差异有统计学意义(均P<0.05)。结论:应用高温高压EDTA修复液可最大化增加Pan的阳性表达强度,达到最佳染色结果,是一种值得推广的免疫组织化学修复方法,可为临床工作提供更好的指导作用。Objective：To explore different conditions and buffers of antigen retrieval which affect the CK of SNIP on immunohistochemical staining results. Method： Dividing paraffin tissue sections of 11 patients into four groups. Using the Image-Pro Plus Image analyzer and taking five horizons for each section to calculate an average of 200 areas, measured standard optical density of the positive reaction areas. Result：It is divided into four groups： high temperature and high pressure citrate buffer retrieval, microwave EDTA buffer retrieval, microwave citrate retrieval, high temperature and high pressure EDTA buffer retrieval. The standard optical density of positive reac- tion areas respectively express： 0. 324 ± 0. 051,0. 325 ± 0. 056,0. 303± 0. 061,0. 365 ± 0. 023. The rates of CK positive expression with high temperature and high pressure EDTA buffer retrieval is batter than other repairing groups in the same paraffin tissue sections（P〈0.05）. Conclusion： For the Pan of Sinonasal inverted papilloma , the method of high temperature and high pressure EDTA buffer antigen retrieval can achieve the ideal staining results？ which is worth while to promote and maybe as a bet？ ter guide of clinic work.

关 键 词：鼻腔鼻窦内翻性乳头状瘤 免疫组织化学 鼠抗人细胞角蛋白(广谱)单克隆抗体 

分 类 号：R739.6[医药卫生—肿瘤]