低氧对牙周膜成纤维细胞增殖和成骨分化的影响  

The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells

作　　者：董家辰[1,2] 宋忠臣[1,2] 束蓉[1,2] 李松[1,2] 林智恺[1,2] 张秀丽[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙周病科 [2]上海市口腔医学研究所,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2014年第4期397-401,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81070838;81271156);上海交通大学医工交叉研究基金(YG2011MS31)~~

摘　　要：目的:观察低氧对体外培养的人牙周膜成纤维细胞(periodontal ligament cells,PDLCs)增殖与成骨分化的影响。方法:体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、100、200、400μmol/L的CoCl2作用于细胞,采用MTT法观察CoCl2对PDLCs增殖的影响,运用实时定量PCR和Western免疫印迹技术检测CoCl2模拟低氧对PDLCs成骨分化的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:免疫组化染色结果证实,培养细胞为牙周膜成纤维细胞。200μmol/L及400μmol/L的CoCl2均能抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原的表达,并呈剂量依赖性。结论:氯化钴模拟的低氧环境对PDLCs增殖及成骨分化具有抑制作用。PURPOSE： To investigate the effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells （PDLCs） in vitro. METHODS： Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of CoCl2 were tested by MTT assay. The PDLCs＇ osteogenic differentiation were investigated using real-time PCR and Western blot. The date was statistically analyzed with SPSS13.0 software package. RESULTS： Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase （ALP）, RUNX2, collagen I were significantly decreased in a dose-dependent manner by 200 μmol/L CoCl2 and 400μmol/L CoCl2. CONCLUSIONS： Hypoxia inhibits proliferation and osteogenic differentiation of human PDLCs.

关 键 词：牙周膜成纤维细胞 低氧 氯化钴 成骨分化 

分 类 号：R781.4[医药卫生—口腔医学]