转录因子RORγτ和FOXP3在口腔扁平苔藓病损组织中的表达及意义  

Expressions of RORγτ and FOXP3 and clinical significance in patients with oral lichen planus

作　　者：谢三祥[1] 丰琳[2] 朱声荣[1] 丁蕾[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030 [2]湖北省黄冈市妇幼保健院检验科,湖北黄冈438000

出　　处：《上海口腔医学》2014年第4期472-476,共5页Shanghai Journal of Stomatology

摘　　要：目的:口腔扁平苔藓(oral lichen planus,OLP)是一种发生于口腔黏膜的T淋巴细胞介导的自身免疫性疾病。辅助性T细胞(helper T lymphocytes,Th)在OLP的发病过程中具有重要作用,本研究旨在进一步探索OLP患者局部病损组织中辅助性T细胞亚群Th17细胞和Treg细胞的作用。方法:纳入43例OLP患者和13例健康志愿者。采用实时定量PCR法检测局部病损组织中Th17和Treg细胞的特征性转录因子RORγτ和FOXP3的表达,采用GraphPad Prism 5软件对其表达差异进行统计学分析。结果:OLP局部病损组织中转录因子RORγτ和FOXP3的表达显著高于正常黏膜组织,而且均与OLP的临床分型密切相关。萎缩糜烂型OLP组病损组织中RORγτ/FOXP3比值显著高于网状型OLP组和健康对照组,而网状型OLP组的RORγτ/FOXP3比值虽然高于对照组,但差异无显著性。结论:Th17细胞和Treg细胞均参与OLP的局部免疫反应;同时,Th17/Treg失衡也参与了重症型OLP的致病过程,且表现为Th17细胞优势。PURPOSE： Oral lichen planus was considered as T cell mediated autoimmune disease affecting oral mucosa with unknown etiopathogenesis. Helper T lymphocytes played an important role in the pathogenesis of OLP. The purpose of this study was to investigate the possible role of Th17 and Treg cells in OLP lesions. METHODS： Forty three patients with OLP（24 patients with reticular OLP and 19 patients with atrophic-erosive OLP） and 13 healthy volunteers were recruited in this study. Real-time quantitative PCR was performed to detect the expressions of RORγτ and FOXP3, which were the lineage-specific transcription factors for Th17 and Treg, respectively. Statistical difference was evaluated by GraphPad Prism 5 software. RESULTS： The results showed that the expressions of RORγτ and FOXP3 in OLP lesions were significantly higher than that in normal oral mucosa, and correlated with the clinical classification of OLP.Additionally, it was found that RORγτ/FOXP3 ratio in atrophic-erosive OLP was significantly higher than that in reticular OLP and control group; Moreover, increased RORγτ/FOXP3 ratio in reticular OLP was found compared with control group,but the difference was not statistically significant. CONCLUSIONS： The results demonstrate that Th17 and Treg cells participate in the immune response in OLP lesions. Th17 predominance of Th17/Treg imbalance may implicate the immune response in atrophic-erosive OLP. These findings help to broaden our view on the pathogenesis of OLP.

关 键 词：FOXP3 RORγτ TH17 TREG 口腔扁平苔藓 

分 类 号：R781.5[医药卫生—口腔医学]