一株高效褐藻酸降解菌的筛选、鉴定及其发酵条件的优化  

The screening, identification of alginate degrading bacteria and optimization of fermentation conditions

作　　者：侯士昌[1] 温少红[1] 唐志红[1] 崔玉琳[2] 秦松[2] 

机构地区：[1]烟台大学生命科学学院,山东烟台264003 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《海洋科学》2014年第7期20-26,共7页Marine Sciences

基　　金：国家自然科学基金资助项目(41176144);海洋公益性行业科研经费资助专项(20120527);山东省自然科学基金项目(JQ200914)

摘　　要：以褐藻酸钠为唯一碳源,从腐烂海带中筛选得到褐藻酸钠降解能力较强的菌株H4,经生理生化和16S rDNA序列分析鉴定该菌株属于交替单胞菌属(Alteromonas)。对菌株H4的发酵条件优化研究表明,H4的较优培养基组成为(w/v):褐藻酸钠0.6%、酵母粉0.5%、蛋白胨0.25%、NaCl 3%;较优培养条件为:培养温度28℃,初始pH7.5,接种量1.5%(v/v),培养时间48 h。Fe3+对交替单胞菌H4的产酶有明显的促进作用,这在其他褐藻酸裂解酶生产菌株中未见报道,而Cu2+对褐藻酸裂解酶的抑制作用高达45.78%。在优化后的培养条件下,粗酶液酶活达到146.45 U/mL,较优化前提高了39.4%。A screening work was carried out to obtain a bacterium with high alginate lyase activity by using alginate as sole source of carbon. The strain was identified asAlteromonas based on physiology and biochemistry analysis, 16S rDNA sequence analysis, and was namedAlteromonassp.H4. It was shown that the optimal culture medium composition of producing alginate lyase was as follows （w/v）： alginate 0.6%, yeast extracts 0.5%, peptone 0.25% and NaCl 3%. The optimized culture condition was as follows： culture temperature 28℃, initial pH value 7.5, inoculum size 1.5%（v/v） and optimal harvest time 48 h. The activity of alginate lyase was activated by Fe^3＋, which hasn’t been reported in other alginate lyase production strains, and the inhibitory effect by Cu^2＋ was up to 45.78%. Under optimized conditions, the alginate lyase activity of the Alteromonas sp. H4 was146.45 U/ml, which was increased by 39.4% than that before optimization.

关 键 词：褐藻酸钠降解菌 筛选 鉴定 酶活优化 

分 类 号：Q935[生物学—微生物学]