孕期感染聚肌胞苷酸大鼠子代海马及额叶星形胶质细胞活化研究  

Astrocyte activation in the prefrontal cortex and hippocampus of schizophrenia rats induced by PolyI: C

作　　者：卢艳梨 李文强[1] 李雪[2] 孟凡翠[1] 丁爽[1] 宋学勤[2] 吕路线[1] 

机构地区：[1]新乡医学院第二附属医院精神科,河南新乡453002 [2]郑州大学第一附属医院精神医学科,河南郑州450052

出　　处：《新乡医学院学报》2014年第9期677-681,共5页Journal of Xinxiang Medical University

基　　金：国家自然科学基金项目(编号:81071090;81371472);河南省基础与前沿研究项目(编号:102300413208;112300413226);河南省科技杰出创新人才项目(编号:124200510019);新乡医学院重点学科开放课题(编号:ZD200991;ZD2011-20)

摘　　要：目的研究聚肌胞苷酸(PolyI:C)孕期感染所致子代精神分裂症大鼠海马及额叶星形胶质细胞活化特点,探讨星形胶质细胞参与神经炎症反应在精神分裂症病理机制中的作用。方法将18只孕鼠随机分为模型组、双倍剂量组和对照组,每组6只。于孕9 d给予尾静脉注射药物,模型组和双倍剂量组分别注射5、10 mg·kg-1PolyI:C,对照组注射等体积(按5 mg·kg-1PolyI:C)生理盐水。待仔鼠8周龄时进行行为学评估,采用免疫组织化学技术检测脑内胶质纤维酸性蛋白(GFAP)的表达。结果模型组和双倍剂量组仔鼠移动总路程与对照组比较差异无统计学意义(P>0.05);共济失调和刻板行为评分大于对照组,差异有统计学意义(P<0.05);模型组与双倍剂量组仔鼠移动总路程、共济失调和刻板行为评分比较差异均无统计学意义(P>0.05)。模型组和双倍剂量组仔鼠T1高于对照组,T2、总反应次数、总条件反射率低于对照组,差异均有统计学意义(P<0.05)。双倍剂量组和模型组仔鼠T1、T2、总反应次数比较差异均有统计学意义(P<0.05);总条件反射率比较差异无统计学意义(P>0.05)。模型组和双倍剂量组仔鼠光密度(OD)值均大于对照组,差异有统计学意义(P<0.05);模型组仔鼠OD值与双倍剂量组比较差异无统计学意义(P>0.05)。结论孕期感染PolyI:C诱导的子代精神分裂症大鼠表现出行为学改变,同时脑内星形胶质细胞存在异常活化,这可能与子代大鼠的行为学改变有关。Objective To study the activation of astrocyte in the frontal cortex and hippocampus of schizophrenia rats induced by PolyI ： C through injecting PolyI： C to pregnant rats and to investigate the role of astrocyte involved in neuroinflammation in the pathogenesis of schizophrenia. Methods Eighteen pregnant rats were randomly divided into model group, double dose group and control group,with six rats in each group. The model group and double dose group were injected with 5, 10 mg · kg^-1 PolyI：C respectively on gestation nine days, and the control group was injected the same volume normal saline. At eight weeks, the offsprings rats of each group were taken behavior tests; the expression of glial fibrillary acid protein（GFAP） in the brain was detected by using immunohistochemistry technique. Results Compared with control group, there were no significant differences of total moving distance in both model group and double dose group （P 〉 0.05 ） ; the scores of ataxia and stereotyped behavior in model group and double dose group were significantly higher than those in control group（P 〈 0.05） ; there were no statistic differences of the total moving distance, scores of ataxia and stereotyped behavior between the model group and double dose group （ P 〉 0.05 ）. Compared with control group, the T1 of model group and double dose group increased, and the T2, the times of total conditioned response, the rate of total conditioned response of the two groups decreased （P 〈 0.05）. There were statistic differences of T1, T2, and times of total conditioned response between the model group and double dose group（P 〈 0. 05 ） ; there was no statistic difference of the rate of total conditioned response between the model group and double dose group（ P 〉0.05 ）. The optical density （OD） value of model group and double dose group was significantly higher than that of control group （P 〈 0.05） ;there was no statistic difference of OD value between the model group and double dose group�

关 键 词：精神分裂症 大鼠 星形胶质细胞 孕期感染 聚肌胞苷酸 

分 类 号：R749.1[医药卫生—神经病学与精神病学]