Lunx mRNA、MMP-7 mRNA对pN0期非小细胞癌淋巴结微转移的诊断及预后的意义  

Detection of micrometastasis and its clinical significance in lymph nodes from pNO non-small cell lung cancer by Lunx mRNA and MMP-7 mRNA

作　　者：常飞云[1] 杨斌[1] 岳强[1] 王春艳[2] 王福花[2] 郭素堂[2] 郑光华[1] 王宏伟[1] 孙诠[1] 

机构地区：[1]山西省肿瘤医院,山西太原030013 [2]山西省肿瘤研究所,山西太原030013

出　　处：《中国医刊》2014年第9期42-45,共4页Chinese Journal of Medicine

基　　金：山西省青年科技研究基金(2011021035-2)

摘　　要：目的应用RT-PCR技术检测pN0期非小细胞肺癌(NSCLC)患者淋巴结肺特异性X蛋白mRNA(Lunx mRNA)、基质金属蛋白酶-7mRNA(MMP-7mRNA)的表达情况,探讨其作为分子标记物对于微转移的诊断价值和临床意义。方法检测41例行根治术的pN0期非小细胞肺癌患者的171枚淋巴结中Lunx mRNA、MMP-7 mRNA的表达。结果15例(36.6%)患者的33枚(19.3%)淋巴结检测存在微转移,这部分患者的TNM分期被重新调整。伴有淋巴结微转移的患者较无微转移的患者有较短的5年生存期(P=0.000)。多因素分析显示,淋巴结微转移、临床分期为影响NSCLC预后的独立危险因素。结论早期诊断区域淋巴结中常规病理检查难以发现的微转移对于NSCLC患者的分子分期、临床治疗和预后判断有着重要的意义。Objective Using real time reverse transcriptase polymerase chain reaction （ RT-PCR） to detect expres-sion of Lunx mRNA and MMP-7 mRNA in lymph nodes from pN0 non-small cell lung cancer（NSCLC） patients, and to discuss its diagnostic value and clinical significance to micrometastasis. Method 171 lymph nodes obtained from 41 cases of pN0 NSCLC going curative operation were examined, and the expression of Lunx mRNA and MMP-7 mRNA were analyzed. Result Micrometastases were detected in 33 lymph nodes （ 19. 3%） from 15 patients （36. 6%）. The TNM staging of these 15 patients was upregulated. The 5-year survival rate of patients with microme-tastasis was significantly lower than that of patients without micrometastasis （P=0. 000）. The results of multivariate analysis confirmed that lymph node micrometastasis and clinical stage were independent prognostic factors. Conclu-sion Early diagnosis of regional lymph node micrometastases that routine pathologic examination is hard to find has important significance in molecular staging, clinical treatment and prognosis judgement for NSCLC patients.

关 键 词：非小细胞肺癌 微转移 聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]