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作 者:邓伟[1] 桂小华[1] 汪桂林[1] 姚杰[1] 闫云君[1]
机构地区:[1]华中科技大学生命科学与技术学院,武汉430074
出 处:《生物技术通报》2014年第9期142-148,共7页Biotechnology Bulletin
基 金:"863"课题(2011AA02A204;2013AA065805)
摘 要:以毕赤酵母发酵废液为水源并提供部分C源和N、P,在500 mL摇瓶中,比较了发酵废液培养基与SE基础培养基对原始小球藻的生长影响,并通过单因素和正交优化发酵废液培养基。结果表明,发酵废液培养基适合于原始小球藻的培养。利用发酵废液培养小球藻,在添加葡萄糖0.05 mol/L,硝酸钠0.01 mol/L,磷酸二氢钾0.003 mol/L,海绿素浓度300μL/L,培养7 d后最高生物量达6.56 g/L,油脂含量达33.68%,两者均高于SE基础培养基。油脂的脂肪酸组成分析表明,废液培养基培养下小球藻油脂的脂肪酸组成主要是C16∶0(25.12%)、C18∶0(4.69%)、C18∶1(50.46%)、C18∶2(6.78%)、C18∶3(8.58%),而SE培养基培养的小球藻油脂脂肪酸组成主要是C16∶0(24.56%)、C18∶0(20.36%)、C18∶1(16.66%)、C18∶2(14.32%)、C18∶3(30.98%),两种培养基培养所得藻油脂肪酸组成虽相差较大,但均适合作为生物柴油的原料。The aim of this article was to examine the feasibility to cultivate Chlorella protothecoides using waste liquor of Pichia pastorisfermentation and then further optimize the cultivation conditions. The results showed that the P. pastoris fermentation waste liquor had more positive effect on the growth of C. protothecoides than SE culture medium. The highest biomass of 0.65 g/L was achieved after 7 days cultivation when using the fermentation waste liquor diluted to 10 times, which was 2.28 fold of that of SE medium. However, it could not significantly improve the lipid content of C. protothecoides. Then, the culture medium was optimized via single factorial experiments and orthogonal experiments. The obtained optimal culture conditions for the maximum biomass were :0.05 mol/L glucose, 0.01 mol/L NaNO3, 0.003 mol/L KH2PO4, and 300 μL/L seaweed liquid fertilizer. Under the optimal culture conditions, 6.56 g/L dry-weight of C. protothecoides biomass and 33.68% lipid content were achieved. The fatty acid profiles of the lipid were C16 :0(25.12%), C18∶0(4.69%), C18∶1(50.46%), C18∶2(6.78%)and C18∶3(8.58%), which were suitable for biodiesel feedstock.
关 键 词:原始小球藻 毕赤酵母发酵废液 生物量 油脂含量 优化
分 类 号:Q949.217[生物学—植物学] X703[环境科学与工程—环境工程]
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