错配修复基因(MMR)蛋白在子宫内膜非典型增生中的表达及其意义  

Expressions of mismatch repair gene(MMR)proteins in atypical endometrial hyperplasia

作　　者：古丽米拉.巴依坦 余丽 师晓莉[3] 张巍[3] 苗娜[3] 

机构地区：[1]新疆人口和计划生育科研所病理科,乌鲁木齐830011 [2]新疆昌吉市第二人民医院病理科,新疆昌吉831100 [3]新疆医科大学第一附属医院病理科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2014年第10期1301-1304,共4页Journal of Xinjiang Medical University

基　　金：新疆医学动物模型研究重点实验室开放课题(XJDX1103-2011-03)

摘　　要：目的探讨子宫内膜非典型增生中错配修复基因(mismatch repair gene,MMR)蛋白MLH1、MSH2、PMS2和MSH6的表达,微卫星不稳定性及意义。方法应用免疫组织化学法检测DNA错配修复蛋白MLH1、PMS2、MSH2和MSH6在子宫内膜非典型增生(50例)及正常增生期宫内膜组织(50例)中的表达,分析微卫星不稳定性与子宫内膜非典型增生临床特征的关系。结果 50例子宫内膜非典型增生组织中MLH1、MSH2、PMS2、MSH6失表达率分别为12%(6/50)、14%(7/50)、12%(6/50)和8%(4/50);MLH1、PMS2、MSH6的表达率高于正常增生期子宫内膜,差异具有统计学意义(P<0.05),两组MSH2的表达差异无统计学意义(P=0.338)。以蛋白失表达情况分组,子宫内膜非典型增生组MSI-H表型7例(14%),MSI-L表型9例(18%),MSS表型34例(68%),与正常增生期宫内膜组比较,差异具有统计学意义(P=0.005)。在子宫内膜非典型增生中,年龄≤50岁者MSI-H明显高于>50岁者(P=0.001)。结论 MMR系蛋白失表达及MSI可能是子宫内膜非典型增生重要分子事件之一。Objective To explore the expressions of mismatch repair gene proteins （MLH1,MSH2,PMS2 and MSH6）,microsatellite instability （MSI）and its significance in atypical endometrial hyperplasia.Meth-ods Immunohistochemical method was adopted to detect the expressions of DNA mismatch repair proteins （MLH1,MSH2,PMS2 and MSH6）in atypical endometrial hyperplasia and in normal prolifera-tive endometrial tissues,and to analyze the relationship between MSI and clinical features of atypical endo-metrial hyperplasia.Results The rates of negative expression of MMR in endometrial atypical hyperplasia were as follows：MLH1 12%（6/50）,MSH2 14%（7/50）,PMS2 12%（6/50）,MSH6 8%（4/50）. Compared with the controls respectively,the differences among MLH1,PMS2 and MSH6 were statistically significant （P 〈0.05）;while there was no significant difference between MSH2 group and others （P =0.338）.Grouped by negative expression, the differences were statistically significant compared with normal proliferative endometrium group （P =0.005 ）.Among them,there were 7 cases of MSI-H （14%）,9 cases of MSI-L（18%）,34 cases of MSS（68%）.MSI-H of cases （≤50 years old）were signif-icantly higher than the others （P=0.001）.Conclusion MSI and the negative expression of MMR proteins may be one of the important molecular events of atypical endometrial hyperplasia.

关 键 词：非典型增生性子宫内膜 微卫星不稳定性 错配修复基因(MMR)蛋白 免疫组织化学 

分 类 号：R365[医药卫生—病理学]