Near and Mid Infrared Spectroscopy to Detect Malolactic Biotransformation of Oenococcus oeni in a Wine-Model  

作　　者：Vigentini Ileana Grassi Silvia Sinelli Nicoletta Di Egidio Valentina Picozzi Claudia Foschino Roberto Casiraghi Ernestina 

机构地区：[1]Department of Food, Environmental and Nutritional Sciences (DEFENS), Milan 20133, Italy

出　　处：《Journal of Agricultural Science and Technology(A)》2014年第6期475-486,共12页农业科学与技术（A）

摘　　要：The aim of this work was to verify the potential of infrared （IR） spectroscopy in near and mid regions to detect the beginning of the malolactic fermentation （MLF） occurring in a model-wine and the further cells autolysis. MLF in wine is a secondary biotransformation due to lactic acid bacteria that usually occurs spontaneou,;ly or after starter inoculation at the end of alcoholic fermentation. Nowadays, it is desirable to supply winemakers with a new rapid and non-destructive approach to monitor MLF progress and 1R spectroscopy technology appears to be suitable for this purpose. The transformation of L-malic acid into L-lactic acid was carried out by inoculating a synthetic wine with an Oenococcus oeni culture and it was monitored through microbiological and chemical methods. At the same time, Fourier transform near infrared （FT-NIR） spectral data, in diffusive transflection mode using an optic probe, and FT-IR spectra, using an germanium crystal attenuated total reflectance （ATR） cell, were collected. Principal component analysis of the spectra was able to identify absorption bands related to the key molecular modifications that took place during the L-malic acid transformation. Thus, the samples were discriminated according to the fermentation phase. Although this study is a preliminary approach, results confirm that near infrared （NIR） and mid infrared （MIR） spectroscopy could be successfully applied to detect the start of MLF and the autolysis of the lactic acid bacteria （LAB） cells.

关 键 词：Malolactic fermentation WINE FT-IR FT-NIR Oenococcus oeni. 

分 类 号：TS207.3[轻工技术与工程—食品科学] TQ921.7[轻工技术与工程—食品科学与工程]