胰腺癌组织中血管内皮生长因子mRNA与尿激酶型纤溶酶原激活物mRNA定量表达的临床意义分析  

Analysis of clinical significance of quantitative expression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue

作　　者：席鹏程[1] 时开网[1] 朱响[1] 杨坤兴[1] 刘子君[1] 卞建民[1] 赵有财[2] 

机构地区：[1]南京医科大学附属南京医院肝胆胰外科,南京210006 [2]南京医科大学附属南京医院病理科,南京210006

出　　处：《临床肝胆病杂志》2014年第8期752-756,共5页Journal of Clinical Hepatology

基　　金：南京市卫生局资助课题(YKK09067)

摘　　要：目的探讨胰腺癌组织中血管内皮生长因子(VEGF)mRNA、尿激酶型纤溶酶原激活物(uPA)mRNA定量表达的临床意义。方法自2008年1月至2011年12月于本科行胰头癌根治术的患者中筛选出经病理证实为导管腺癌的30例患者的完整资料,采用荧光定量PCR(qPCR)检测其胰腺癌组织及6例正常胰腺组织中VEGF mRNA、uPA mRNA定量表达,分析其与临床病理因素之间的关系。结果 VEGF mRNA、uPA mRNA的表达与胰腺癌的组织分化程度、神经侵犯有关。VEGF mRNA在淋巴结转移阳性组中的定量表达高于淋巴结转移阴性组,两组比较差异有统计学意义(t=20.007,P=0.000);uPA mRNA在直径≤2 cm的肿瘤组织中定量水平小于直径>2 cm的肿瘤组织,两组比较差异有统计学意义(t=7.539,P=0.000)。uPA mRNA在伴有十二指肠侵犯组中的定量表达高于无十二指肠侵犯组,两组比较差异有统计学意义(t=-2.089,P=0.037)。uPA mRNA在Ⅲ期肿瘤组织中的定量表达高于Ⅰ、Ⅱ期肿瘤组织中的定量表达,两组比较差异有统计学意义(t=-9.450,P=0.000)。VEGF mRNA与uPA mRNA的表达存在正相关(r=0.334,P=0.000)。结论 VEGF mRNA、uPA mRNA在胰腺癌组织中过度表达可为肿瘤细胞的侵润创造条件。Objective To investigate the clinical significance of quantitative expression of vascular endothelial growth factor （VEGF）mR-NA and urokinase-type plasminogen activator （uPA）mRNA in pancreatic cancer tissue.Methods A retrospective study was conducted on the complete data of 30 patients with a pathological diagnosis of duct adenocarcinoma who were selected from those treated by radical re-section of pancreatic head carcinoma from January 2008 to December 2011.Real-time quantitative PCR was used to measure the quantita-tive expression of VEGF mRNA and uPA mRNA in the pancreatic cancer tissues of the 30 cases and the normal pancreatic tissues of 6 con-trols,and its relationship with clinicopathological factors was analyzed.Results The quantitative expression of VEGF mRNA and uPA mR-NA was correlated with the histological differentiation and perineural invasion of pancreatic cancer.The quantitative expression of VEGF mR-NA was significantly higher in patients with lymphatic metastasis than in those without lymphatic metastasis group （t=20.007,P=0.000）. The quantitative expression of uPA mRNA was significantly lower in the tumors with a diameter of≤2 cm than in the tumors with a diameter of 〉2 cm （t=7.539,P=0.000）.Patients with duodenal invasion had a significantly higher quantitative expression of uPA mRNA than those without duodenal invasion （t=-2.089,P=0.037）.The quantitative expression of uPA mRNA in stage III tumor tissues was signifi-cantly higher than that of uPA mRNA in stage I and II tumor tissues （t=-9.450,P=0.000）.There was a positive correlation between VEGF mRNA expression and uPA mRNA expression （r=0.334,P=0.000）.Conclusion The overexpression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue may create an environment that enables the invasion by pancreatic cancer cells.

关 键 词：胰腺肿瘤 血管内皮生长因子A 尿纤溶酶原激活物 基因表达 

分 类 号：R735.9[医药卫生—肿瘤]