凋亡相关蛋白在激素性幼兔股骨头缺血坏死动物模型中的表达及意义  

Expression and significance of apoptosis-related proteins in steroid induced by juvenile rabbit models with avascular necrosis of femoral head

作　　者：罗宇[1] 俞松[1] 王健[1] 唐诗鹏[1] 陆建国[1] 闫陶然 卓金伟 

机构地区：[1]遵义医学院附属医院小儿矫形外科,563003

出　　处：《中华实用儿科临床杂志》2014年第17期1349-1351,共3页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的观察各凋亡相关蛋白在激素性幼兔股骨头缺血坏死动物模型股骨头组织中的表达情况，探讨该模型中调控凋亡的主要通路。方法选用2月龄的新西兰大白兔，制作糖皮质激素性幼兔股骨头缺血坏死模型及对照组模型，根据是否发病将激素注射组分为未发病组和发病组。取股骨头软骨及软骨下骨组织用免疫组织化学法检测凋亡通路中凋亡相关蛋白天冬氨酸特异酶切的半胱氨酸蛋白酶-3（Caspase-3）、Caspase-8、人结合凋亡蛋白酶活化因子-1（apaf-1）、钙蛋白酶-1（calpain-1）的表达情况。分别测定在单位视野中Caspase-3、Caspase_8、apaf-1、calpain-1的积分光密度（IOD）值。结果1．Caspase-3的IOD值分别为发病组25142．72±21528．48，未发病组2069．63±1096．96，对照组301．80±99．66。Caspase-8的IOD值分别为发病组24942．63±18942．99，未发病组2016．31±1518．70，对照组236．85±97．94。Apaf-1的IOD值分别为发病组8514．23±6384．20，未发病组1118．49±1360．59，对照组95．13±38．05。Calpain-1的IOD值分别为发病组9636．71±9123．81，未发病组1881．10．4-3277．86，对照组126．71±47．35。Caspase-3在发病组和未发病组、对照组间差异有统计学意义（H=11．470、23．996，P＜0．01）；Caspase-8在发病组和对照组间差异有统计学意义（H=22．178，P＜0．01）；apaf-1在发病组和对照组间差异有统计学意义（H=22．808，P＜0．01）；calpain-1在发病组和对照组间差异有统计学意义（H=13．553，P＜0．01）。2．线性回归分析：Caspase-8能够显著的预测Caspase-3，且回归方程回归效应显著，回归方程能够解释40．3％的变异，而apaf-1和calpain-1对Caspase-3的回归效应不显著。结论凋亡受体通路可能在股骨头缺血坏死的凋亡过程中发挥主要调控作用。Objective To detect the apoptosis of femoral head cartilage cells and to observe the expression of apoptosis-related proteins in the tissues of the femoral head, as well as to explore the main pathway for regulating the apoptosis in steroid induced by juvenile rabbit models with avascular necrosis of femoral head. Methods The models with avascular necrosis of the femoral head and the control group model were made in New Zealand infancy albino rabbits induced by glucocorticoid（GC）. The immunohistochemical method was used to detect the expressions of Caspase- 3,Caspase-8,apoptosis protease activating factor-1 （ apaf-1 ） ,calpain-1 in the femoral heads. Results 1. The integrated optical density（IOD） values of Caspase-3 in GC-indueed subgroup,the subgroup that was not induced by GC and control group were 25 142.72 ± 21 528.48,2 069.63 ± 1 096.96 and 301.80 ±99.66, respectively. The IOD values of Caspase-8 in GC-induced subgroup, the subgroup respectively and the control group were 24 942.63 -＋ 18 942.99, 2 016.31 ± 1 518.70,236.85 ± 97.94,respectively. The IOD values of apaf-1 in GC-induced subgroup, the subgroup that was not induced by GC and the control group were 8 514.23 ±6 384.20,1 118.49± 1 360.59,95.13 ± 38.05 ,respectively. The IOD values of ealpain-1 in GC-induced subgroup,the subgroup that was not induced by GC and control group were 9 636.71 ± 9 123.81 , 1 881.10 ±3 277.86,126.71±47.35, respectively. The IOD value differences of the Caspase-3 between GC-induced subgroup and the subgroup that was not induced by GC,the control group were extremely significant（H = 11. 470,23. 996, P 〈0.01 ）. The IOD value differences of the Caspase-8, apaf-1, calpain-1 between GC-induced subgroup and the control group were extremely significant （H = 22. 178,22. 808,13. 553, P 〈0.01 ）. 2. The linear regression analysis results showed that under the joint action of Caspase-8, apaf-1, calpain-I ,only the Caspase-8 could significantly predict Caspase-3, and its regression equation regression g

关 键 词：凋亡相关蛋白 股骨头缺血坏死 动物模型 幼兔 糖皮质激素 

分 类 号：R-332[医药卫生]