三种抗褐化剂对南荻外植体褐变及愈伤诱导率的影响  

Effect of Three Antioxidants on Browning of Explant and Callus Induction Rate of Miscanthus lutarioriparius

作　　者：夏亚男 蒋建雄[1] 易自力[1] 刘清波[1] 黄红梅[1] 陈智勇[1] 

机构地区：[1]湖南农业大学芒属植物研究所,湖南长沙410128

出　　处：《北方园艺》2014年第17期93-96,共4页Northern Horticulture

基　　金：国家"863"科技支撑计划区域重大专项资助项目(2011AA10020901);教育部高校博士点基金资助项目(20124320110007)

摘　　要：以能源植物南荻的幼穗为外植体,MS为基本培养基,通过在诱导培养基中分别添加一定浓度梯度的聚乙烯吡咯烷酮(polyvinylpyrrolidone,PVP)、维生素C(vitamin C)及柠檬酸(citric acid,CA),对比研究3种抗褐化剂对南荻外植体褐变及愈伤诱导率的影响。结果表明:在诱导培养基中,添加PVP、维生素C和CA均能减轻外植体的褐化程度;相对于对照(CK)35.83%的诱导率,在试验浓度范围内,添加PVP、维生素C和CA后愈伤组织诱导率可分别提高至55.42%、50.42%和124.00%,CA处理组与对照组之间愈伤诱导率差异达极显著水平(P<0.01)。因此,CA为南荻幼穗愈伤组织诱导的最佳抗褐化剂,且浓度为0.2g/L时诱导率显著提高2.46倍。Taking the immature inflorescences of M.Lutarioriparius as explants and MS medium with polyvinylpyrrolidone（PVP）,vitamin C or citric acid（CA）were used as medium in this research,the effect of3 antioxidants to inhibit browning of explants and on callus induction rate of Miscanthus lutarioriparius were studied.The results showed that treating with PVP,vitamin C and CA could improve callus induction rate and reduce the browning of explants in a certain degree.Compared with the control whose callus induction rate were 35.83%,adding PVP,vitamin C or CA in the medium increased the induction rate to 55.42%,50.42%and 124.00%respectivly;and adding CA made highly significant difference（P〈0.01）.CA was the best anti-browning agent of immature inflorescences culture of M.lutarioriparius,and 0.2g/L CA could significantly increase the callus induction rate by 2.46 times.

关 键 词：南荻 组织培养 褐化 聚乙烯吡咯烷酮 抗坏血酸 柠檬酸 

分 类 号：Q813.12[生物学—生物工程]