维生素E对二甲基甲酰胺致小鼠慢性肝损伤的预防作用  

Preventive effect of vitamin E on chronic liver injury induced by N,N-dimethylformamide in mice

作　　者：张秀梅[1] 姜超[1,2] 于素芳[1] 

机构地区：[1]山东大学公共卫生学院劳动卫生与环境卫生学研究所,山东济南250012 [2]济南市市中区疾病预防控制中心,山东济南250022

出　　处：《中国药理学与毒理学杂志》2014年第4期515-518,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：山东省科技发展计划(2012GSF11854)~~

摘　　要：目的探讨维生素E(Vit E)对二甲基甲酰胺(DMF)致小鼠慢性肝损伤的预防作用。方法将40只健康SPF级雄性昆明小鼠随机分为正常对照组、Vit E对照组、DMF染毒模型组和DMF+Vit E干预组,每组10只。DMF染毒模型组和DMF+Vit E干预组ig给予DMF0.5 g·kg-1染毒;2 h后,Vit E对照组和DMF+Vit E干预组ig给予Vit E 5 mg·kg-1,每天1次,连续30 d。用试剂盒测定小鼠血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)和黄嘌呤氧化酶(XOD)活性及丙二醛(MDA)含量,HE染色法观察肝组织病理改变。结果与正常对照组比较,Vit E对照组小鼠上述各指标均无显著变化,DMF染毒模型组小鼠血清中GOT,GPT和XOD活性及肝组织MDA含量均升高(P<0.05,P<0.01),且肝组织存在明显的病理损伤,肝细胞核固缩,细胞水肿、变性、坏死。与DMF染毒模型组比较,DMF+Vit E干预组小鼠血清GOT,GPT和XOD活性及肝组织MDA含量均降低(P<0.05,P<0.01),肝组织病理损伤明显减轻,几乎看不到变性和水肿细胞。结论 Vit E对DMF引起的小鼠慢性肝损伤具有一定的预防作用。OBJECTIVE To study the preventive effect of vitamin E（Vit E）on chronic Iiver injury in-duced by N,N-dimethylformamide（ DmF）. METHODS Healthy male Kunming mice were randomly divided into 4 groups,10 mice in each group. The mice in DmF model group and DmF＋Vit E group were ig given DmF 0.5 g·kg-1 ,while Vit E controI group and DmF＋Vit E group were ig given Vit E 5 mg·kg-1 after 2 h once a day for 30 d. The activity of serum glutamic-oxaloacetic transaminase（GOT）,glutamic-pyruvic transaminase（GPT）and xanthine oxidase（XOD）and the content of malondialdehyde（mDA） in Iiver tissue were measured with corresponding kits. The pathoIogical changes of Iiver tissue were ob-served with HE staining. RESULTS Compared with the normal control group,the activity of serum GOT,GPT and XOD of the DmF model group was significantly elevated（P﹤0.05,P﹤0.01）,and the Iiver cell nucleus pycnosis,cellular edema,degeneration,necrosis occurred. Compared with DmF model group,the activity of serum GOT,GPT and XOD of DmF＋Vit E group were all significantly reduced（P﹤0.05,P﹤0.01）,the content of mDA in Iiver tissue was significantly diminished（P﹤0.01）,but histopatho-Iogical changes of Iiver tissue were significantly improved. CONCLUSlON Vit E has preventive effect on chronic Iiver damage induced by DmF through antioxidation.

关 键 词：二甲基甲酰胺 维生素E 肝损伤 

分 类 号：R575.3[医药卫生—消化系统]