卡铂碳包铁纳米笼壳聚糖纳米球靶向治疗大鼠移植性肝癌的初步研究  

Effects of targeted treatment of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles on rats with transplanted liver cancer

作　　者：郭跃华[1] 鲍世韵[1] 严文辉[1] 周汉新[1] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院肝胆外科,518020

出　　处：《肿瘤研究与临床》2014年第7期433-436,共4页Cancer Research and Clinic

基　　金：广东省自然科学基金（04006966）;深圳市科技计划重点项目（200601016）

摘　　要：目的 观察卡铂碳包铁纳米笼壳聚糖纳米球（C-Fe@CN-CN）对大鼠移植性肝癌的靶向治疗效果.方法 将24只移植性肝癌大鼠按数字表法随机分为4组,每组6只,A组注入0.9％NaCl溶液,为对照组;B组注入卡铂0.9％NaCl溶液;C组注入C-Fe@CN-CN 0.9％NaCl溶液分散液,肿瘤部位不施加磁场;D组注入C-Fe@CN-CN 0.9％NaCl溶液分散液,肿瘤部位施加磁场30 min.开腹行肝动脉插管给药,给药后第7天,再次开腹,完整切除肿瘤,测量肿瘤质量和体积,计算瘤重抑制率.留取肿瘤标本,观察肿瘤病理变化和纳米球沉积情况.结果 移植性肝癌大鼠接受不同剂型卡铂治疗后,肿瘤质量和体积的增长均被显著抑制.A、B、C、D组的肿瘤质量分别为（0.85±0.12）、（0.61±0.10）、（0.48±0.09）、（0.33±0.06）g,差异有统计学意义（P＜0.05）;B、C、D组的瘤重抑制率分别为28.9％、43.4％、61.7％.A、B、C、D组的肿瘤体积分别为（1.06±0.24）、（0.72±0.10）、（0.50±0.07）、（0.28±0.05） cm3,差异有统计学意义（P＜0.05）.D组6只大鼠肿瘤组织病理切片显示肿瘤组织重度坏死,坏死组织中有C-Fe@CN-CN相互聚集;C组5只中度坏死,1例重度坏死;B组4只中度坏死,2例轻度坏死;A组6只均为轻度坏死.结论 C-Fe@CN-CN在体内对大鼠移植性肝癌具有优越的靶向治疗效果.Objective To investigate the effects of targeted treatment of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles （C-Fe@CN-CN） combining external magnetic field on rats with transplanted liver cancer.Methods Twenty-four model rats with transplanted liver cancer were established and divided into four groups randomly （n =6）.Abdominal exposure was carried out through a midline incision,and a cannula was inserted into the hepatic artery and fixed.Group A：saline water was injected as control,group B：saline water with 10 mg/kg free carboplatin was given,group C：saline water with C-Fe@CN-CN （equivalent dose of free carboplatin 10 mg/kg） was injected in absence of magnetic field,group D：saline water with C-Fe@CN-CN （equivalent dose of free carboplatin 10 mg/kg） was injected in presence of magnetic field for 30 min.All the animals were sacrificed and abdominal exposure was done again after 7 days.After tumors were reselcted,tumor weight and volume was measured,the inhibiting rate of tumor weight was calculated.Tumor and liver tissues were examined for histological changes.Results The growth of tumor was significantly inhibited after therapy with different forms of carboplatin.There was significant difference in the tumor weight of A,B,C,D groups [（0.85±0.12） g,（0.61±0.10） g,（0.48±0.09） g,（0.33±0.06） g,P ＜ 0.05,respectively].The inhibiting rates of tumor weight of B,C,D groups were 28.9 ％,43.4 ％,61.7 ％ respectively.The inhibiting rate of D group was highest which was 1.1 times higher than that of B group.There was also significant difference in the tumor volume of A,B,C,D groups [（1.06±0.24） cm3,（0.72±0.10） cm3,（0.50±0.07） cm3,（0.28±0.05） cm3,P ＜ 0.05,respectively].The tumor volume of group A was largest which was 2.8 times larger than that of group D.In group D,tumor tissues from six rats presented severe necrosis,and nanoparticles were concentrated in the necrotic tissue.In group C,five rats presented middle necrosis,one rats presented seve

关 键 词：卡铂 磁靶向 分子靶向治疗 纳米球 肝肿瘤 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]