外周T细胞淋巴瘤非特指型TCRβ V/J取用及CDR3谱型分析  

Analysis of the usage of TCRβ V/J subfamily and CDR3 repertoire in patients with peripheral T-cell lymphoma unspecified

作　　者：张建波[1] 宋永平[2] 于庆凯[1] 吕晓东[3] 胡骏[4] 

机构地区：[1]郑州大学附属肿瘤医院河南省肿瘤医院病理科,450003 [2]郑州大学附属肿瘤医院河南省肿瘤医院血液科,450003 [3]郑州大学附属肿瘤医院河南省肿瘤医院中心实验室,450003 [4]中山大学中山医学院微生物学教研室

出　　处：《白血病．淋巴瘤》2014年第8期465-467,共3页Journal of Leukemia & Lymphoma

基　　金：河南省基础与前沿技术研究计划（102300410038）

摘　　要：目的 分析外周T细胞淋巴瘤非特指型TCR β V/J亚家族限制性取用及CDR3谱型.方法 提取4例外周T细胞淋巴瘤非特指型患者病变淋巴结总RNA后进行反转录,多重聚合酶链反应（PCR）扩增TCRβ全长序列,构建重组质粒并测序,利用网上TCR资源分析序列.结果4例患者共获得9个TCR β链全长序列.克隆性增生T细胞V、J亚家族分别限制性取用BV2、BV4S2、BV14、BV29S1和BJ1S4、BJ2S3、BJ2S5、BJ2S7,并具有不同的CDR3区氨基酸序列.结论外周T细胞淋巴瘤非特指型TCRV β、VJ亚家族具有限制性取用,不同T细胞克隆的CDR3序列不同.Objective To analyze the restricted usage of TCRβ V/J subfamily and CDR3 repertoire in patients with peripheral T-cell lymphoma unspecified （U-PTL）.Methods The total RNA was respectively extracted from lymph node of U-PTL and reverse transcriptase,then multi-PCR was used to amplify the complete DNA sequence（CDS） of TCR β-chain.The recombinant plasmids were sequenced and sequence was analyzed by using online TCR resources.Results There were 9 TCR β chain CDS obtained from four patients.TCRβ-chain presented specific repertoire skewing in patients with U-PTL.There were restricted usage of BV2,BV4S2,BV14,BV29S1 of BV subfamily and BJ1S4,BJ2S3,BJ2S5,BJ2S7 of BJ subfanily.The clonal proliferation T cells had different CDR3 amino acid sequences.Conclusions There are restricted usage of TCR β V/J subfamily in patients with U-PTL.The sequences of CDR3 in different TCR clone proliferation are mostly different.

关 键 词：T细胞淋巴瘤 多重PCR 互补决定区 

分 类 号：R733.1[医药卫生—肿瘤]