灵芝多糖对单核巨噬白血病细胞株的体外作用  

The Dual Effects of Ganoderma Lucidum Polysaccharides on Monocyte-Macrophage Leukemia Cell Lines In Vitro

作　　者：李宁[1] 赵树立[2] 徐贞俊[2] 翁磊华[2] 侯亚义[2] 

机构地区：[1]济南大学医学与生命科学学院,山东济南250022 [2]南京大学医学院&南京大学国家生物医药技术重点实验室,江苏南京210093

出　　处：《现代生物医学进展》2014年第27期5238-5241,共4页Progress in Modern Biomedicine

基　　金：济南大学博士基金(XBS1346)

摘　　要：目的：研究灵芝多糖对单核巨噬白血病细胞THP-1和RAW264.7的肿瘤生物学活性的影响。方法：用1、50和100μg/ml的灵芝多糖和脂多糖（LPS）刺激THP-1和RAW264.7细胞,CCK-8法测定巨噬细胞的增殖活力、流式细胞仪检测细胞的凋亡活性、Q-PCR检测细胞因子TNF-α、IL-1、IL-6和IL-12基因、相关凋亡信号通路基因TRADD、TNFSF10、TNFRSR10b、NFκBI、Caspase10和Caspase 3基因的mRNA表达水平。结果：灵芝多糖可以呈反浓度依赖性地刺激两种细胞的增殖,在50和100μg/ml浓度下可引起细胞凋亡,凋亡信号基因TNFRSR10b和NFκBI的mRNA水平在24h升高了53%和48%;在1~100μg/ml浓度下可上调细胞因子TNF-α、IL-1、IL-6和IL-12细胞因子mRNA的表达水平。结论：灵芝多糖对白血病细胞株具有双重作用,在低浓度下促进细胞增殖,而在高剂量时诱导细胞凋亡,均可上调免疫相关细胞因子的表达。Objective： To study the effects of Ganoderma lucidum polysaccharides（GLP） on tumor biology of the monocyte-macrophage THP-1 and RAW264.7. Methods： After THP-1 and Raw264.7 cells were stimulated with different concentrations of GLP and LPS, the proliferation and apoptosis activity of cells were analyzed by CCK-8 kits and flow cytometry. RT-PCR was used to detect expression of cytokines of macrophages,such as TNF-α, IL-1, IL-6 and IL-12, and Q-PCR was used to detect the gene expression levels in apoptosis signaling pathway. Results： GLP could promote proliferation of THP-1 and RAW264.7 cells in an inverse dose-dependent, and up-regulate expression of cytokines secretion at all concentrations. And also GLP could elevate expression of apoptosis related genes TNFRSR10 b and NFκBI. Conclusion： GLP played a dual role on leukemia cell line, that is it increased expression of cytokines and promoted cells proliferation at lower concentration, while at high doses caused cells apoptosis.

关 键 词：灵芝多糖 单核巨噬白血病细胞 死亡受体信号通路 细胞因子 

分 类 号：R733.7[医药卫生—肿瘤]