周细胞覆盖率与胃癌侵袭相关性的分析  

Analysis of the Relationships between Microvessel Pericyte Coverage Index and Invasion in Gastric Cancer

作　　者：韩伟[1] 翟丽丽[2] 王正彩[3] 吴红梅[3] 齐蕾[4] 周秋锋[5] 单丽辉 柴翠翠 王瑞芬[8] 管雯斌[8] 王立峰[8] 

机构地区：[1]哈尔滨医科大学附属第一医院病理科,黑龙江哈尔滨150001 [2]齐齐哈尔市第一医院病理科,黑龙江齐齐哈尔161000 [3]哈尔滨医科大学附属第四医院病理科,黑龙江哈尔滨150001 [4]河北省邢台市人民医院病理科,河北邢台054031 [5]浙江省湖州市安吉县人民医院病理科,浙江湖州313000 [6]哈尔滨市第五医院病理科,黑龙江哈尔滨150000 [7]深圳市坪山区人民医院病理科,广东深圳518118 [8]上海交通大学医学院附属新华医院病理科,上海200092

出　　处：《现代生物医学进展》2014年第28期5446-5450,共5页Progress in Modern Biomedicine

基　　金：黑龙江省卫生厅科研课题(2010-029)

摘　　要：目的:探讨周细胞在胃癌血管周围的覆盖率是否可以作为判断胃癌预后的一个指标。方法:利用免疫组织化学双染的方法同时标记内皮细胞(抗CD34抗体)和周细胞(抗α-SMA抗体),检测48例胃癌石蜡标本当中微血管壁周细胞覆盖率(microvessel pericyte coverage index,MPI),应用SPSS11.5统计软件分析MPI与胃癌患者性别、年龄、肿瘤大小、浸润深度、淋巴结转移、临床分期、以及脉管内是否有癌栓等临床病理参数之间的关系。结果:MPI与胃癌的分化程度呈正相关(P=0.002),与脉管内是否有癌栓呈负相关(P=0.002),而与患者年龄、性别、肿瘤大小、浸润深度、临床分期、淋巴结转移等无关(P均>0.05)。结论:利用免疫组织化学双染的方法可以清晰标记胃癌的微血管结构,血管的不成熟即周细胞覆盖率低成为胃癌患者预后差的重要因素。Objective： To investigate whether Microvessel pericyte coverage index whether can be used as an indicator to determine the prognosis of gastric cancer. Methods： Forty-eight surgical specimens from our department were used. Double staining of endothelial cells with anti-CD34 antibody and pericytes with anti-a-smooth muscle actin antibody was performed. The microvessel pericyte coverage index （MPI） as an index of microvessel maturation was evaluated. All statistical analyses were performed with SPSS11. 5, including MPI relations to the gender, age, size, the depth, lymph node metastasis, clinical stages and cancer embolus in the tube. Results： MPI had a positive correlation with the extent of cancer differentiation （P=0.002） and a negative correlation with vascular invasion （P=0.002）. But MPI had nothing to do with other characteristics of pathology, such as gender, age and lymph node metastasis. Conclusions： To utilize double-staining with immunohistochemical technology can clearly mark the structure of the microvessels. The immature microvessels, which means low MPI is an important factor to the poor prognosis of gastric cancer.

关 键 词：胃癌 肿瘤血管发生 周细胞 周细胞覆盖率 

分 类 号：R737.31[医药卫生—肿瘤]