TPM1在鼻咽癌中的表达及其临床意义  

TPM1 expression and its clinical significance in nasopharyngeal carcinoma

作　　者：王亮[1] 林建立[1] 

机构地区：[1]深圳大学医学部,518060

出　　处：《中国现代药物应用》2014年第19期17-19,共3页Chinese Journal of Modern Drug Application

摘　　要：目的观察TPM1基因的表达与鼻咽癌(NPC)的关系。方法采用实时定量rt-PCR技术检测TPM1基因在NPC细胞株(CNE1、CNE2、58F)和鼻咽上皮细胞株(NP69)中的表达;采用组织芯片技术通过免疫组化检测TPM1在124例恶性鼻咽癌肿瘤、31例对应的癌旁正常组织和12例正常鼻咽黏膜组织的表达。结果 TPM1 mRNA在NP69中表达水平较高,而在NPC细胞株中表达水平显著下降。正常鼻咽黏膜及癌旁组织中TPM1蛋白染色阳性表达率约为65.1%(28/43);而在鼻咽癌组织中阳性表达率约为34.7%(43/124);TPM1蛋白表达水平与肿瘤的侵犯范围(T)呈负相关(P<0.05),与颈淋巴结转移(N)无关(P>0.05)。结论 TPM1基因表达的抑制可能参与鼻咽癌的发生。Objective To investigate the relation between TPM1 gene expression and nasopharyngeal carcinoma （NPC）. Methods TPM1 expression was examined in three NPC cell lines （CNE1, CNE2 and 58F） and one nasopharyngeal epithelial cell line （NP69） by real time rt-PCR analysis and in tissue array with 124 specimens of malignant NPC, 31 NPC specimens adjacent areas and 12 normal mucosa tissues by immunohistochemistry staining. Results Compared to NP69, TPM1 mRNA expression level is significantly lower in three NPC cell lines. The positive rates of TPM1 protein were 65.1% （28/43） in NPC specimens adjacent areas and normal mucosa tissues and were 34.7% （43/124） in malignant NPC tissues. The expression of TPM1 protein in NPC was negatively related to that in degree of infiltration （T） （P〈0.05） but not related to that in lymph node metastasis （N） （P〉0.05）. Conclusion Down-regulated of TPM 1 expression may be associated with the tumorigenesis of NPC.

关 键 词：TPM1 鼻咽癌 基因表达 

分 类 号：R739.63[医药卫生—肿瘤]