中国大陆地区基因1a亚型丙型肝炎病毒系统进化分析  

Phylogenetic analysis of subgenotype 1a of hepatitis C virus in China's Mainland

作　　者：杨松[1] 鞠威[2] 冯胜虎[2] 全敏[2] 王琦[2] 刘顺爱[2] 邢卉春[1] 成军[1,2] 

机构地区：[1]首都医科大学附属北京地坛医院肝病中心,北京100015 [2]首都医科大学附属北京地坛医院传染病研究所,北京100015

出　　处：《中华实验和临床感染病杂志（电子版）》2014年第4期5-7,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：中国肝炎防治基金会光辉基金资助项目(No.GHF20100207);北京市优秀人才培养资助D类项目(No.2012D003034000030);首都卫生发展科研专项项目(No.首发2011-2017-02)

摘　　要：目的明确我国大陆地区基因1a亚型丙型肝炎病毒系统进化的情况。方法收集慢性丙型肝炎患者的血清标本,采用逆转录-巢式PCR扩增HCV基因组部分NS5B区和(或)部分核心/E1区片段。根据测序结果构建系统进化树,分析大陆地区基因1a亚型丙型肝炎病毒系统进化情况。结果共收集全国23个中心共1 012例患者标本,其中970例标本分型成功,基因1a亚型患者4例。以美国国立卫生院丙型肝炎数据库中不同国家来源丙型肝炎病毒序列为参考构建系统进化树成功。系统进化分析提示,4株HCV 1a亚型部分NS5B区序列散在分布于进化树中的不同分支,来源于不同国家。结论基因1a型HCV由国外传播入我国,在中国丙型肝炎病毒感染者中所占比率低,在大陆地区随机流行。Objective To investigate the phylogeny profile of subgenotype l a of hepatitis C virus in China's Mainland. Methods Serum samples and clinical data were collected from patients with chronic hepatitis C from 23 sites in China's Mainland. NSSB and/or Cote/El region for sequencing were amplified by RT-nested PCR. Phylogenetic tree which built with reference sequences from NIH HCV database and phylogeny profile of subgenotype la of hepatitis C virus in China's Mainland were analyzed. Results Serum sample and clinical data were collected from 1 012 enrolled patients with CHC. HCV subgenotype were identified in 970/1 012 patients. Total of 4 patients with subgenotype la were identified and phylogenetie tree were built with reference sequence for NIH HCV database. Phylogenetic analysis showed these 4 sugenotype la NSSB region sequences originated from different countries and scattered in different branches in the phylogenetie tree. Conclusions Subgenotype la of HCV in China's Mainland originated from abroad. The proportion of subgenotype la in all Chinese HCV subgenotypes is low and subgenotype la patients are scattered in China's Mainland, randomly.

关 键 词：肝炎病毒 丙型 基因型 系统进化分析 

分 类 号：R512.63[医药卫生—内科学]