不同工艺对芎麻汤成分质量浓度及配比的影响  

Determination and ratio of main ingredient in Xiongma decoction by different extraction process

作　　者：赵瑛[1] 刘琳[1] 黄坤杰[1] 李蔚[1] 王亚贤[2] 李伦[1] 

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076 [2]黑龙江中医药大学,哈尔滨150088

出　　处：《哈尔滨商业大学学报（自然科学版）》2014年第4期392-394,425,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省教育厅项目(12511134)

摘　　要：比较不同提取工艺所得芎麻汤中阿魏酸、天麻素的质量浓度及其配比关系,为芎麻汤的新药创制及现代复方中药的质量控制、主效应的确定及配伍规律研究提供科学依据.分别采用煎煮法、不同体积分数乙醇回流提取法提取川芎、天麻,制成芎麻汤,HPLC法测定阿魏酸和天麻素的质量浓度,并计算其配比.阿魏酸色谱条件为流动相:甲醇-0.01%冰醋酸溶液(30∶70,V/V),检测波长:310nm;天麻素色谱条件为流动相:乙腈-0.05%磷酸水溶液(3∶97,V/V),检测波长:220 nm.芎麻汤水提液阿魏酸质量浓度0.976 mg/mL,天麻素1.586 mg/mL,阿魏酸与天麻素的配比为1∶1.6;芎麻汤乙醇回流提液中阿魏酸质量浓度6.08 mg/mL,天麻素3.57 mg/mL.传统方剂制备工艺改变,可能带来方剂主效应的改变,主要有效成分配比应纳入质量考察.The determination and ratio of ferulic acid and gastrodin in Xiongma decoction ex-tract by different extraction process were compared to provide scientific research basis on cre-ating new drugs , controlling the quality control of modern compound traditional Chinese med-icine, determining the main effect and compatibility .Chuanxiong and Tianma were extracted by different extraction method such as the boiling method and ethanol extraction of different concentrations to make Xiongma decoction .The content of ferulic acid and gastrodin were determined by HPLC and their ratio was calculated .Chromatographic conditions of ferulic acid were as follows： mobile phase： methanol -0.01% acetic acid solution （30∶70, V/V）, the detection wavelength：310 nm.Chromatographic conditions of Gastrodin were as fol-lows：mobile phase：acetonitrile-0.05% phosphoric acid （3∶97, V/V）, detection wave-length：220 nm.The determination of ferulic acid was 0.976 mg/mL,gastrodin was 1.586 mg/mL, the ratio of ferulic acid and gastrodin was 1∶1.6 in boiling method;while the deter-mination of ferulic acid was 6.08 mg/mL, gastrodin was 3.57 mg/mL, the ratio was 1.7∶1 in ethanol extraction .There were different determination and ratio , when there was differ-ent extraction process in the same prescription .This may bring about changes the main effect in prescription .This suggested that the ratio of the main active ingredient should be included in the quality inspection , when preparation process of the traditional recipe was changed .

关 键 词：芎麻汤 提取工艺 阿魏酸 天麻素 高效液相色谱 

分 类 号：R283[医药卫生—中药学]