黄酮衍生物HT6的抗炎作用及机制初步研究  

Mechanism of flavonoids HT6 in mouse inflammatory model

作　　者：蒋美玲[1] 缪明星[1] 龚国清[1] 

机构地区：[1]中国药科大学药理教研室,南京210009

出　　处：《中南药学》2014年第8期755-758,共4页Central South Pharmacy

摘　　要：目的研究黄酮衍生物(HT6)对小鼠炎症模型的抗炎作用机制。方法 ICR雄性小鼠随机分为7组,正常组和模型组给予0.5%CMC-Na溶液,萘普生组给予120 mg·kg-1的萘普生,原黄酮化合物组每日给予100 mg·kg-1的原黄酮化合物,HT6低、中、高剂量组分别给予25、50、100 mg·kg-1的HT6,3 d后,通过小鼠耳廓肿胀和足肿胀模型来观察HT6的抗炎作用,眼眶取血,测炎症因子前列腺素E2(PGE2)及白三烯B4(LTB4)的含量。结果 HT6高、中剂量组能明显抑制耳廓和足肿胀度以及LTB4的含量(P<0.05),HT6高剂量组能明显降低PGE2的含量(P<0.05)。结论 HT6有一定的抗炎作用,作用机制可能是通过下调致炎因子PGE2和LTB4的表达水平来实现的。Objective To determine the mechanism of fl avonoids（HT6） in different mouse infl ammatory models.Methods Totally 112 male ICR mice were divided into 6 groups randomly： a normal group（0.5% CMC-Na）, a model group（0.5% CMC-Na）, a naproxen group（120 mg·kg- 1）, an original fl avonoids group [100 mg/（kg·d）] and a HT6 low group [25 mg/（kg·d）], a HT6 medium group [50 mg/（kg·d）] and a HT6 high group [100 mg/（kg·d）]. After 3 days of intragastric administration, mouse ear edema, mouse paw edema, and ELISA were used to assess the levels of infl ammation and serum cytokines PGE2 and LTB4. Results The experiment suggested that the high and medium HT6 group decreased the ear edema and mouse paw edema（P〈0.05）, and reduced serum LTB4 level（P〈0.05）. The high HT6 group also reduced PGE2 expression（P〈0.05）. Conclusion HT6 has anti-infl ammatory effect and the mechanism may be the down-regulation of PGE2 and LTB4 levels.

关 键 词：黄酮衍生物 抗炎作用 耳廓肿胀 足肿胀 PGE2 LTB4 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]