MCPH1增强宫颈癌caski细胞对顺铂敏感性的作用及机制  

Effect and mechanism of MCPH1 on enhancing cisplatin chemosensitivity in cervical cancer caski cell

作　　者：李韵[1] 麦力[1] 张冀[1] 马冬梅[1] 李海玉[1] 樊建军[1] 宋方洲[1] 

机构地区：[1]重庆医科大学分子医学与肿瘤研究中心,重庆400016

出　　处：《中国免疫学杂志》2014年第9期1169-1173,共5页Chinese Journal of Immunology

基　　金：青年科学基金项目(No.30800410)

摘　　要：目的:研究MCPH1加强宫颈癌caski细胞对顺铂敏感性的作用及机制。方法:将慢病毒转染并筛选好的MCPH1过表达组caski+,和对照组caski-,分别用顺铂(cisplatin)处理,MTT法检测生长抑制效率,DAPI染色观察细胞凋亡形态,流式细胞仪检测细胞凋亡率,Real-time PCR检测相关基因Bax、Caspase-3和P53 mRNA表达。结果:caski+cddp+组的生长抑制作用比caski-cddp+相更强,并呈时效和量效关系;DAPI染色形态学显示caski+cddp+组凋亡小体增多,可见致密强荧光;流式细胞仪检测凋亡率结果为caski+cddp+组凋亡率比caski-cddp+组高13.21%,其中晚期凋亡更明显,caski+cddp+组比caski-cddp+组高17.16%;Real-time PCR检测基因Bax、Caspase-3的mRNA水平,caski+cddp+组比caski-cddp+组分别高出2倍和77%,caski+cddp+组抑癌基因P53的mRNA水平也有上升。结论:MCPH1能增强宫颈癌caski细胞对顺铂的敏感性,为MCPH1的进一步研究和宫颈癌的化疗耐药性研究奠定了基础。Objective：To study the effect and mechanism of MCPH1 on enhancing cisplatin chemosensitivity in cervical cancer caski cell. Methods： Cisplatin acted on MCPH1 overexpression group caski＋ cell which was transfected with lentiviral and filtered, and control group caski- cell , respectively. The cell-growth inhibition was measured by MTT assay. Apoptotic morphology was analyzed with DAPI. Apoptosis rate was detected by flow cytometry. The mRNA expressions of related genes, such as Bax , Caspase-3 and P53, were quantified by Real-time PCR. Results： Growth inhibition of group caski＋cddp＋ was more obvious than group caski-cddp＋ in a time- and dose-dependant manner. DAPI staining morphology displayed that,caski＋ cddp＋ group had more apoptotic bodies and dense strong fluorescence. The apoptosis rate detected by flow cytometry of group caski＋cddp＋ was 13.21% higher than group caski-cddp＋. Among them,late stage apoptosis of group caski＋cddp＋ was more obvious ,group caski＋cddp＋ was 17.16% higher than group caski- cddp＋. The mRNA expressions quantified by Real-time PCR of Bax and Caspase-3 in group caski＋cddp＋ was twice and 77% higher than group caski- cddp ＋ respectively. The mRNA level of tumor suppressor gene P53 in group caski ＋ cddp ＋ also increased. Conclusion： MCPH1 could enhance cisplatin chemosensitivity in cervical cancer caski cell, which may provide the foundation for further research of MCPH1 and study of chemoresistance in cervical cancer.

关 键 词：MCPH1 宫颈癌 顺铂 敏感性 

分 类 号：R737.33[医药卫生—肿瘤]