抗骨增生片对兔膝骨关节炎软骨组织病理形态及IGF-1,p38MAPK表达的影响  

Effect of Kangguzengsheng tablet on pathomorphlogy and expressions of IGF- 1 and p38MAPK of cartilage in rabbits with KOA

作　　者：陈守中[1] 郑云生[1] 王志文[2] 王国权[1] 

机构地区：[1]唐山职业技术学院,河北唐山063000 [2]河北联合大学,河北唐山063000

出　　处：《现代中西医结合杂志》2014年第28期3093-3095,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的观察抗骨增生片对兔膝骨关节炎软骨组织病理形态及胰岛素样生长因子1(IGF-1)、p38丝裂原活化蛋白激酶(p38MAPK)表达的影响。方法将40只成年雄性大耳白兔随机分为正常组(A组)10只和造模组30只。膝关节腔内注射1.6%木瓜蛋白酶,造成兔膝骨关节炎模型。造模成功后,再将造模组随机分为模型组(B组)、抗骨增生片低剂量组(C组)、高剂量组(D组)各10只,C、D组分别灌胃0.36,0.72 g/(kg·d)的抗骨增生片,1次/d;A、B组每天灌胃等量生理盐水。4周后,取膝关节软骨组织,观察软骨组织病理形态变化,用免疫组化法检测软骨组织中IGF-1、p38MAPK的表达情况。结果 C组、D组软骨组织破坏程度较B组明显减轻(P均<0.01);C组、D组IGF-1的表达较B组显著升高(P均<0.05),p38MAPK的表达较B组显著降低(P均<0.01)。结论抗骨增生片能抑制兔骨关节炎软骨病理损害,机制可能与其上调IGF-1、下调p38MAPK的表达,抑制过度的软骨细胞凋亡有关。Objective It is to observe the effect of Kangguzengsheng tablet on pathomorphlogy and expression of IGF - 1 and p38 mitogen-activated proteikinase （p38MAPK）in cartilage of the rabbit of knee osteoarthritis （KOA）. Methods 40 healthy male rabbits were randomly divided into normal control group （group A, n = 10） and model control group （n = 30）. The KOA rabbit models were induced by injection 1.6% papain into the cavum articulare. After the models were successfully established, the model control group was randomly divided into model group （ group B） , low dosage of Kangguzengsheng tablet group （group C） and high dosage group （group D） , each group had 10 rabbits. Group C and group D were respectively given Kangguzengsheng tablet 0.36, 0. 72 g/（ kg· d） once a day by intragastric administration, and group A and group B was given the same dosage of normal saline. After 4 weeks, cartilage tissue of knee joint was gotten to observe the pathomorphlogy changes, and the expression of IGF - 1 and p38MAPK were detected by immunohistochemistry technique. Results The cartilage damage in group group C and group D was obviously relieved compared with that in group B （ P 〈 0.01 ）. The expression of IGF - 1 was significantly higher while the expression of p38MAPK was lower in group C and group D than that in group B（ P 〈 0.01 or P 〈 0.05 ）. Conclusion Kangguzengsheng tablet can inhibit the pathomorphlogy damage of joint cartilage in rabbits, and its mechanism may be that it can increase the expression of IGF - 1 and reduce the expression of p38MAPK, and inhibit excessive cartilage cell apoptosis.

关 键 词：抗骨增生片 骨关节炎 软骨组织 胰岛素样生长因子1 P38丝裂原活化蛋白激酶 

分 类 号：R-332[医药卫生]