大豆GmNCED1基因的克隆及表达模式分析  

Cloning and expression analysis of GmNCED1 from Glycine max

作　　者：李琼琼[1] 张洁[1] 邓宇[1] 于威君 高红桃[1] 靳京[1] 王南[2] 王法微[2] 李海燕[2,1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118

出　　处：《中国油料作物学报》2014年第4期455-460,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31271746;31201144;31101091);教育部高等学校博士学科点专项科研基金(20122223120003);吉林省发展与改革委员会项目(JF2012C002-4)

摘　　要：本研究以抗逆性强的大豆旱碱一号为材料,首次从中克隆了编码9-顺式-环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase,NCED)的GmNCED1基因全长cDNA片段,该基因编码区含有1 836个核苷酸,编码611个氨基酸。通过同源性比对分析发现GmNCED1与花生、菜豆等双子叶豆科植物NCED的同源性高达84%以上。同时,利用荧光定量PCR分析发现高盐、低温、干旱、外源ABA以及NAA处理均可以诱导该基因在大豆叶片及根中表达。本研究初步揭示GmNCED1基因在植物逆境胁迫中的作用,为基因工程育种提供优质的候选基因。Soybean （Glycine max）is one of the important oil crops in the world.The yield of soybean is strongly affected by environmental stress such as salinity and dehydration.Here we cloned the 9-cis-epoxycarote-noid dioxygenase gene （named GmNCED1 ）from soybean （Hanjian 1 ）which had stronger adaptability and toler-ance to abiotic stresses.The open reading frame of GmNCED1 was 1 836 bp,encoding 6 1 1 amino acids.Sequence analysis and homology comparison of GmNCED1 showed that it had highest homology with dicotyledonous plants （peanuts,pea,etc）with more than 85% homologies.At the same time,we analyzed the expression profiling of GmNCED1 during various abiotic stresses using qRT-PCR.The result showed that the expression of GmNCED1 could be induced by salt,drought,NAA,and ABA treatments in leaves and roots.This study explained the func-tion of GmNCED1 in abiotic stresses and provided a candidate gene for genetically engineered crops.

关 键 词：大豆 基因克隆 表达分析 荧光定量PCR 

分 类 号：S565.103[农业科学—作物学]