虫草发酵液胞外多糖的醇沉工艺优选  

Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps

作　　者：陈丽华[1] 张清华[1] 管咏梅[1] 程磊[1] 李钰[1] 朱卫丰[1] 杨明 吴德智[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,南昌330004 [2]江西济民可信金水宝制药有限公司,南昌330096

出　　处：《中国实验方剂学杂志》2014年第18期20-22,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-30);江西省落地计划项目(赣财教指[2013]71号);江西中医药大学研究生创新专项资金立项项目(JZYC12A01);江西省研究生创新专项资金立项项目(YC2013-S238)

摘　　要：目的:优选冬虫夏草发酵液中胞外多糖的醇沉工艺参数。方法:采用苯酚-硫酸法测定胞外多糖含量,检测波长489.4 nm。以胞外多糖质量为指标,通过正交试验考察乙醇用量、醇沉温度和时间对发酵液中胞外多糖的水提醇沉工艺的影响。结果:最佳醇沉工艺参数为加4倍量95%乙醇于20℃醇沉7.5 h;胞外多糖质量0.671 g,纯度69.32%。结论:发酵液中胞外多糖含量较高,可加以充分利用;优化的虫草胞外多糖发酵液醇沉工艺稳定可行。Objective：To optimize alcohol precipitation process of extra-cellular polysaccharides in fermentative fluid of Cordyceps.Method：Phenol-sulfuric acid was adopted to determine the content of extracellular polysaccharides with detection wavelength at 489.4 nm.Taking the content of extra-cellular polysaccharides as index,orthogonal test was adopted to optimize alcohol precipitation process with ethanol amount,alcohol precipitation temperature and time as factors.Result：Optimum alcohol precipitation process parameters were as follows：alcohol precipitation for 7.5 h with 4 times the amount of 95 ％ ethanol at 20 ℃ ; the content of extra-cellular polysaccharides was 0.671 g with purity of 69.32％.Conclusion：The content of extracellular polysaccharides in fermentative fluid of Cordyceps is high and can be utilized sufficiently.This optimized alcohol precipitation is reasonable and practical for industrial production of Jinshuibao capsules.

关 键 词：虫草发酵液 胞外多糖 苯酚-硫酸比色法 金水宝 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]