Haplotype variation of Green Revolution gene Rht-D1 during wheat domestication and improvement  

作　　者：Chihong Zhang Lifeng Gao Jiaqiang Sun Jizeng Jia Zhenglong Ren 

机构地区：[1]Agronomy Department, Sichuan Agricultural University [2]Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture [3]Chengdu Institution of Biology, theChinese Academy of Sciences

出　　处：《Journal of Integrative Plant Biology》2014年第8期774-780,共7页植物学报（英文版）

基　　金：supported by the National Basic Research Program of China (2010CB125905, 2010CB951501);National Transgenic Major Project (2013 08009-001)

摘　　要：Green Revolution made a substantial contribution to wheat yields worldwide in the 196os and 197os. It is of great importance to analyze the haplotype variation of Rht-D1, the Green Revolution gene, during wheat （Triticum aestivum L.） domestication and breeding to understand its evolution and function in wheat breeding history. In this study, the Rht-D1 and its flanking regions were sequenced and single nucleotide polymorphisms were detected based on a panel of 45 accessions of Aegilops tauschii, 51 accessions of landraces and 8o accessions of commercial varieties. Genetic diversity in the wild accessions was much higher than that in the varieties and higher than that reported previously. Seven haplotypes （Hapl I to Hapl VII） of Rht-D1 were identified and their evolutionary relationships were proposed. In addition to the well-known Green Revolution allele Rht-Dlb, Hapl Vii （an allele Rht-Dlk） was identified in early breeding varieties, whichreduced plant height by 16%. The results suggested that Rht-Dlk had been used in breeding before the Green Revolution and made a great contribution to wheat production worldwide. Based on the breeding history and molecular evidence, we proposed that the wheat Green Revolution in China and International Maize and Wheat Improvement Center （CIMMYT） occurred independently.Green Revolution made a substantial contribution to wheat yields worldwide in the 196os and 197os. It is of great importance to analyze the haplotype variation of Rht-D1, the Green Revolution gene, during wheat （Triticum aestivum L.） domestication and breeding to understand its evolution and function in wheat breeding history. In this study, the Rht-D1 and its flanking regions were sequenced and single nucleotide polymorphisms were detected based on a panel of 45 accessions of Aegilops tauschii, 51 accessions of landraces and 8o accessions of commercial varieties. Genetic diversity in the wild accessions was much higher than that in the varieties and higher than that reported previously. Seven haplotypes （Hapl I to Hapl VII） of Rht-D1 were identified and their evolutionary relationships were proposed. In addition to the well-known Green Revolution allele Rht-Dlb, Hapl Vii （an allele Rht-Dlk） was identified in early breeding varieties, whichreduced plant height by 16%. The results suggested that Rht-Dlk had been used in breeding before the Green Revolution and made a great contribution to wheat production worldwide. Based on the breeding history and molecular evidence, we proposed that the wheat Green Revolution in China and International Maize and Wheat Improvement Center （CIMMYT） occurred independently.

关 键 词：DIVERSITY HAPLOTYPE Rht-D1 single nucleotidepolymorphism WHEAT 

分 类 号：S512.1[农业科学—作物学] X38[环境科学与工程—环境工程]