SNaPshot技术检测茶树SNP研究  

Study of SNaPshot Detect SNP Markers in Tea Plant

作　　者：张成才[1] 谭礼强[1,2] 王丽鸳[1] 韦康[1] 成浩[1] 

机构地区：[1]中国农业科学院茶叶研究所 国家茶树改良中心,浙江杭州310008 [2]四川农业大学园艺学院,四川雅安614025

出　　处：《茶叶科学》2014年第2期180-187,共8页Journal of Tea Science

基　　金：现代农业产业技术体系建设专项(No.nycytx-23);浙江省自然科学基金(X307603);浙江省茶产业技术创新战略联盟专项

摘　　要：为了提高茶树 SNPs 分型效率，促进 SNPs 在茶树遗传育种中的应用，研究了 SNaPshot 技术进行茶树SNPs分型的可行性。从实验室前期确证的SNPs中，选择10个作为目标SNPs；使用SNaPshot技术在不同的茶树品种中进行分型；然后，对分型结果进行比对和统计，以验证 SNaPshot 技术检测茶树 SNPs 的准确性、重复性以及在茶树遗传多样性分析等方面的可用性。结果发现，6个 SNPs分型结果与测序结果一致，准确率为60%；目标SNPs在龙井43及其重复实验中的分型结果完全一致；6个SNPs的等位基因数都是2，期望杂合度（He）介于0.37～0.52，观测杂合度（Ho）介于0.32～0.74，多态性信息含量（PIC）介于0.36～0.50；结果表明，SNaPshot技术对茶树SNPs的分型准确性高、重复性好，可以用于茶树遗传多样性分析以及茶树遗传图谱构建等方面的研究。In order to increase the genotyping efficiency of tea SNPs and promote th e application of SNPs in tea genetic breeding investigation, the feasibility of SNaPshot in SNPs genotyping of tea plant was investigated. Ten SNPs were selected from previous experiment as target SNPs. Then, these SNPs were detected in different tea cultivars by SNaPshot technology. Six among 10 SNPs were successful detected, with an accuracy of 60%. The polymorphism diversity of these SNPs was also analyzed. The value of NA was 2, He ranged from 0.37 to 0.52, Ho ranged from 0.32 to 0.74, PIC ranged from 0.36 to 0.50. All these markers were shown coincide betweenLongjing43and its repeated test. The markers reported here will be useful for tea genetic linkage map construction and genetic diversity study. The SNaPshot technology will promote the genetic study and accelerate the breeding process in tea plant.

关 键 词：茶树 遗传多样性 遗传图谱 

分 类 号：S571.1[农业科学—茶叶生产加工] Q52[农业科学—作物学]