海洛因成瘾对大鼠海马CA3区NR1、NR2A亚基表达的影响  

The effects of expression of NR1,NR2A subunit in heroin addicted rats of hippocampus CA3 area

作　　者：肖邦[1,2] 潘贵书[1] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003 [2]川北医学院生理学教研室,四川南充637000

出　　处：《国际检验医学杂志》2014年第17期2275-2276,共2页International Journal of Laboratory Medicine

摘　　要：目的观察海洛因依赖大鼠海马CA3区NMDA受体NR1、NR2A亚基的表达。方法将40只SD大鼠随机分成海洛因依赖组、海洛因+MK801组、海洛因戒断组及对照组,用逆转录聚合酶链反应(RT-PCR)技术半定量检测海马NMDA受体NR1、NR2A亚基mRNA的表达。结果与对照组比较,海洛因依赖组、海洛因戒断组和海洛因+MK801组的NR1表达明显升高(P<0.05),且海洛因依赖组NR1的表达明显高于海洛因戒断组和海洛因+MK801组,海洛因戒断组与海洛因+MK801组的NR1表达差异无统计学意义(P>0.05)。与对照组比较,海洛因+MK801组NR2A的表达降低(P<0.05),海洛因戒断组NR2A表达升高(P<0.05),依赖组NR2A的表达差异无统计学意义(P>0.05)。结论海洛因依赖可使大鼠海马CA3区NR1的表达明显上调。海洛因戒断的大鼠海马CA3区NR2A表达上调,使用MK801干预的大鼠海马CA3区NR2A表达下调。Objective To observe the effects of expression of NMDA receptor NR1 ,NR2A subunit in heroin dependent rats of hippocampus CA3 area.Methods 40 SD rats were randomly divided into four groups：Heroin Addiction Group,Heroin＋MK801 Group,Heroin Abstinence Group and Control Group.The expression of NMDA receptor NR1 subunit mRNA were semi-quantita-tively determined by reverse transcription-polymerase chain reaction （RT-PCR）.Results Compared with Control Group,the NR1 expression in the other three groups significantly increased（P〈0.05）.NR1 expression in Heroin Addiction Group was higher than Heroin＋MK801 Group and Heroin Abstinence Group（P〈0.05）.There was no statistically significant difference between Heroin＋MK801 Group and Heroin Abstinence Group（P〉0.05）.Compared with Control Group,Heroin＋MK801 Group′s NR2A ex-pression reduced（P〈0.05）,Heroin Abstinence Group′s NR2A expression increased（P〈0.05）,no significantly difference was ob-served in Heroin Addiction Group（P〉0.05）.Conclusion Heroin addiction causes NR1 expression increase in hippocampus CA3 area of rats.The CA3 area NR2A expression ascended in Heroin Abstinence Group but reduced in the Heroin rats administrated with MK801 .

关 键 词：海洛因成瘾 海马 NMDA受体 大鼠 

分 类 号：R749.6[医药卫生—神经病学与精神病学]