莲心碱对膀胱癌细胞T24增殖及其周期的调控  

Regulation of liensinine on T24 bladder cancer cell proliferation and cycle

作　　者：闫亓 张天禹[1] 谭宁[2] 徐宾[1] 徐伶俐[3] 刘晓佳[4] 

机构地区：[1]桂林医学院附属医院泌尿外科,广西桂林541004 [2]桂林医学院科学实验中心,广西桂林541004 [3]桂林医学院附属医院消化内科 [4]附属医院病理科,广西桂林541004

出　　处：《重庆医学》2014年第25期3268-3271,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81360377)

摘　　要：目的通过莲心碱作用于膀胱癌细胞T24,研究其对膀胱癌细胞T24增殖的影响。方法使用不同浓度的莲心碱处理膀胱癌细胞T24,通过CCK-8实验及克隆形成实验,检测莲心碱对细胞增殖的影响;对细胞进行PI染色后,使用流式细胞仪,检测莲心碱对T24细胞周期的影响;通过实时定量PCR,检测莲心碱作用后T24细胞的p21基因mRNA的改变。结果莲心碱对膀胱癌细胞T24增殖有明显的抑制作用,各剂量组(1.562 5、3.125 0、6.250 0、12.500 0、25.000 0μg/mL)与甲醇对照组差异有统计学意义(P<0.05),并呈剂量依赖性;细胞周期检测结果显示,莲心碱将T24细胞阻滞在S期;实时定量PCR检测结果显示,莲心碱提高T24细胞p21基因的mRNA。结论莲心碱对膀胱癌细胞T24具有增殖抑制并将其阻滞在S期,该作用机制可能与p21表达上调有关。Objective To study the effect of liensinine on the proliferation of human bladder cancer T24 cells.Methods T24 cells were treated with different concentrations of liensinine.Its influence on the cell proliferation was detected by the CCK-8 exper-iment and the clonogenic experiment.After staining of T24 cells,the influence of liensinine on the cell cycle was examined by the flow cytometry.The mRNA change of p2 1 gene was determined by real-time quantitative PCR.Results Compared with the con-trol,liensinine significantly inhibited the proliferation of T24 cells in different doses groups（1.562 5,3.125 0,6.250 0,12.500 0, 25.000 0μg/mL）,the differences had statistical significance and showed the dose-dependence;the cell cycle detection results re-vealed that liensinine arrested the T24 cells at the S phase;the real-time quantitative PCR detection results showed that liensinine increased mRNA of p21 gene in T24 cells.Conclusion Liensinine inhibits the proliferation of T24 bladder cancer cells and arrests the T24 cells at S phase,its mechanism may be related with the upregulation of p21 expression.

关 键 词：莲心碱 膀胱肿瘤 细胞增殖 细胞周期 P2 1 

分 类 号：R737.14[医药卫生—肿瘤]