双酚A对小鼠囊胚发育和植入的影响  

Effects of Bisphenol-A on Blastocyst Development and Implantation

作　　者：晏佩佩 潘晓燕[1] 王红荷 李质馨[1] 王雪楠[2] 赖勤[1] 宋文静[1] 赵华一 窦肇华[1] 

机构地区：[1]吉林医药学院组织学与胚胎学教研室,吉林132013 [2]济宁医学院附属医院生殖医学中心,山东济宁272029

出　　处：《中国医学科学院学报》2014年第4期351-356,共6页Acta Academiae Medicinae Sinicae

基　　金：吉林省教育厅"十二五"科学技术研究项目[吉教科合字(2013)第349号];山东省自然科学基金(ZR2011HL005);吉林医药学院大学生科研基金[吉医学科字(2011)第08号;吉医学科字(2012)第04号]和吉林省大学生创新创业训练计划项目[(2013)第850号]~~

摘　　要：目的 研究双酚A （BPA）对囊胚发育和植入的影响.方法 对妊娠鼠进行完全随机化分组,分为100、300、600 mg/（kg·d）BPA组和对照组,将BPA处理组的孕鼠从妊娠的0.5～3.5 d灌服100、300和600 mg/（kg·d）BPA,着床点染色,统计胚胎的着床数和着床率,检查植入前囊胚的发育和孵化情况,激光扫描共聚焦显微镜观察孵化囊胚的卵裂球分布和含半胱氨酸的天冬氨酸蛋白水解酶3的表达.结果 300 mg/（kg·d）BPA显著降低了孕鼠的着床点数和着床率,而600 mg/（kg·d）BPA处理的孕鼠未观察到着床点,抑制了胚胎的植入.不同浓度的BPA对妊娠第4天囊胚发育率和囊胚总细胞数无影响,但300和600 mg/（kg·d）BPA显著降低了囊胚的孵化率,提高了孵化囊胚中凋亡细胞的数量.结论 高浓度的BPA损伤了附植前囊胚的发育,影响了胚胎的植入.Objective To determine the effects of bisphenol-A （BPA） on blastocyst development and implantation.Methods According to completely randomized grouping method,90 pregnant mice were divided into 100,300,and 600 mg/（kg · d） BPA groups and control group.BPA-treated pregnant mice were orally administered with BPA at concentrations of 100,300 and 600 mg/（kg · d） from day 0.5 to day 3.5 of their pregnancy.Blastocyst implantation and development were studied.Results In the 300 mg/（kg · d） BPA group,the number of implantation sites and implantation rate were significantly decreased.In the 600 mg/（kg · d） group,no implantation sites were observed among pregnant mice and BPA inhibited embryo implantation.Blastocyst development on day 4 was examined,and findings showed that the development rate and total numbers of blastocysts in BPA treatment groups had no significant difference from the control group.However,BPA at 300 and 600 mg/（kg · d） significantly reduced blastocyst hatching rate and dramatically increased the number of blastocyst apoptotic cells when compared with those in the control group.Conclusion BPA at a high concentration damages the blastocyst development before implantation and inhibits embryo implantation.

关 键 词：双酚A 囊胚发育 胚胎植入 凋亡 

分 类 号：R339.2[医药卫生—人体生理学]