硫化氢对大鼠局灶性脑缺血/再灌注损伤的保护作用及其机制  

Protective effect of hydrogen sulfide on focal cerebral ischemia /reperfusion injury in rats and its mechanism

作　　者：李新娟[1] 魏林郁[1] 李超堃[1] 卢娜[1] 王国红[1] 赵红岗[1] 李东亮[1] 

机构地区：[1]河南省新乡医学院生理学与神经生物学教研室,河南新乡453003

出　　处：《中国药理学通报》2014年第9期1271-1275,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81371346;81271376);河南省教育厅科学技术研究重点项目(No 14A310019);新乡医学院优秀硕士资助课题

摘　　要：目的探讨硫化氢(H2S)对大鼠局灶性脑缺血/再灌注损伤的保护作用及其机制。方法♂SD大鼠随机分成3组:假手术组、脑缺血/再灌注(I/R)组和硫氢化钠(NaHS)+I/R。线栓法建立大鼠左侧大脑中动脉栓塞(MCAO)模型,缺血2 h,再灌注24 h后,计算各组死亡率、Longa评分标准进行神经功能缺陷评分,2,3,5-三苯基氯化四氮唑(TTC)染色测量脑梗死体积,免疫荧光法检测大脑皮质和海马组织中P2X7受体蛋白表达。结果 NaHS+I/R组大鼠死亡率(27.27%)明显低于I/R组(42.86%),该组大鼠神经功能缺陷评分也明显低于I/R组(P<0.05),且脑梗死体积(21.88%±3.53%)明显低于I/R组(36.71%±3.73%)(P<0.01)。免疫荧光结果显示,与假手术组相比,I/R组大脑皮质、海马CA1区P2X7阳性表达细胞数明显增多(P<0.01);与I/R组相比,NaHS+I/R组大脑皮质、海马CA1区P2X7阳性表达细胞数明显减少(P<0.01)。结论 H2S可对局灶性脑缺血/再灌注损伤大鼠发挥脑保护作用,其机制可能与下调P2X7受体蛋白表达有关。Aim To investigate the protective effects of hydrogen sulfide （ H2 S） on focal cerebral ischemia/reperfusion injury in rats and the possible mechanisms. Methods Male Sprague Dawley rats were divided into three groups randomly： sham-operated group, cerebral ischemia/ reperfusion （ I/R） group and sodium hydro-sulfide （ NaHS ） ＋ I/R group. The left temporary middle cerebral artery occlusion （ MCAO ） model was established by the line-embolism method. After rats were suffered 2h/24h ischemia/reperfusion stress, the mortality rate was evaluated, and the nervous function-al defect degree was evaluated by Longe scoring, the volumes of cerebral infarction was evaluated by 2 ,3 ,5-triphenyltetrazolium chloride （ TTC） staining, and the expression of P2X7 receptor protein in brain tissue was detected by the immunofluorescence method. Results The mortality rate in NaHS ＋ I/R rats （ 29.41%） was obviously lower than those of I/R group （ 42 . 86%） . The nervous defect scores in NaHS ＋ I/R rats were significant lower than those of I/R group （ P 〈0.05 ） . The volumes of cerebral infarction in NaHS ＋I/R group （21.88% ±3.53%） were significant lower than those of I/R group （ 36.71% ±3.73%） （ P 〈0.01 ） . The results of immunofluorescence showed that the positive expression cells of P2X7 receptor protein in cerebral cortex and hippocampal CA1 area of I/R group were significantly higher than those of sham-op-erated group（P〈0. 01）. However, compared with I/R group, the positive expression cells of P2X7 receptor protein in cerebral cortex and hippocampal CA1 area of NaHS ＋ I/R group were significantly decreased （ P〈0. 01）. Conclusions H2S exerts the neuroprotective effect on focal cerebral ischemia/reperfusion injury in rats, and the protective mechanism might be associated with down-regulating the expression of P2X7 receptor protein in brain tissue.

关 键 词：硫化氢 脑缺血 再灌注损伤 P2X7受体 TTC染色 大鼠 保护作用 

分 类 号：R-332[医药卫生] R322.81