松果菊苷对阿尔采末病模型大鼠学习、记忆功能及氧自由基水平的影响  

Effect of echinacoside on learning-memory ability and oxygen free radicals on model rats with Alzheimer's disease

作　　者：丁慧[1] 陈虹[1] 姜勇[2] 屠鹏飞[2] 马婧怡[1] 张万鑫[1] 

机构地区：[1]石河子大学药学院教育部新疆特种植物药资源重点实验室,新疆石河子832002 [2]北京大学中医药现代研究中心,北京100083

出　　处：《中国药理学通报》2014年第9期1302-1305,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30860334,30560171)

摘　　要：目的研究松果菊苷(ECH)对Aβ25-35引起的阿尔采末病(AD)大鼠的学习与记忆能力的改善和氧自由基水平的影响。方法 60只SD大鼠,体质量(300±10)g,随机分为假手术组、模型组、ECH高、中、低剂量组(40、20、10 mg·kg-1·d-1)、阳性药石杉碱甲组(Hup-A,0.02 mg·kg-1·d-1)。水迷宫实验评价其学习记忆能力的改变。检测大脑皮质和海马中丙二醛(MDA)、一氧化碳(NO)的含量和超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性。结果 Aβ25-35能够严重损害大鼠学习记忆能力;与模型组大鼠相比,ECH不同剂量治疗组均能够减少其对学习记忆能力的损害(P<0.01,P<0.05)。ECH治疗组大鼠大脑皮质和海马组织中的MDA含量明显下降,SOD的活性明显升高,明显减少NO、NOS的释放(P<0.01,P<0.05)。结论 ECH可以改善AD大鼠学习记忆力,其作用机制可能与加快氧自由基的清除能力和降低大鼠脑内氧化应激水平有关。Aim To investigate the effects of echina-coside （ ECH ） on the learning and memory capacities and brain level of oxygen free radicals of rats with de-mentia induced by amyloid β-peptide. Methods Six-ty Sprague Dawley rats, weighing （300±10） g, were randomly divided with 10 rats pergroup into 6 groups：sham operated group, model, ECH high dose （40 mg ·kg-1·d-1）, ECH middle dose （20 mg· kg-1· d-1）, ECH low dose （10 mg·kg-1·d-1） and Hup A （Huperzine A, 0. 02 mg·kg-1·d-1） group. Mor-ris maze tests were conducted for evaluating the learn-ing and memory ability. Content of malondialdehyde （MDA）, nitric oxide （NO） and activities of superox-ide dismutase （ SOD） and NO synthase （ NOS） in the hippocampus and cortex were detected. ResultsAβ25-35 induced significant learning and memory im-pairment in the rats. Compared with the rats in model group, those treated with ECH at different doses all manifested alleviation of learning and memory impair-ment （ P〈0 . 01 , P〈0 . 05 ） . Cotents of MDA of ECH treatment group were obviously decreased, while SOD activities were obviously increased, and significantly reduced the release of NO and NOS in the hippocam-pus and cortex brain tissue （ P 〈0 . 01 , P 〈0.05 ） . Conclusion ECH can enhance the learning and mem-ory ability in rats with AD, which is presumably relat-ed to accelerating the cleaning of oxygen free radicals and reducing oxidative stress in brain of AD rats.

关 键 词：松果菊苷 阿尔采末病 水迷宫 AΒ25-35 氧自由基 海马组织 大脑皮层 

分 类 号：R-332[医药卫生] R284.1