桂枝汤对4-甲基邻苯二酚诱导心脏交感神经芽生的抑制作用  

Inhibition effect of Guizhi decoction on cardiac sympathetic sprouting

作　　者：李晓[1] 姜萍[2] 林海青[1] 徐向东[1] 杨金龙[2] 马度芳 于潇华[2] 姜月华[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]山东中医药大学临床学院,山东济南250355

出　　处：《中国药理学通报》2014年第9期1320-1325,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81072962)

摘　　要：目的观察桂枝汤对4-甲基邻苯二酚(4-MC)诱导的心脏交感神经的抑制作用。方法 10 mg·L-1的4-MC(10μg·kg-1体重)腹腔注射30d诱导建立心脏交感神经芽生模型,分别给予美托洛尔和不同桂芍比例的桂枝汤(桂芍比分别为1∶1、1∶2、2∶1)进行药物干预。观察大鼠心率、心电图改变,ELISA法检测左心室和右心房心肌匀浆中去甲肾上腺素(NE)、生长相关蛋白(GAP-43)、酪氨酸羟化酶(TH)、乙酰胆碱酶(CHAT)的含量,免疫荧光法观察右心房、左心室中酪氨酸羟化酶(TH)阳性神经纤维的分布。结果 4-MC造成心脏交感神经芽生,对CHAT无影响。与模型对照组比较,各药物组心率降低;各组心电图无明显差异;相比模型对照组,各药物组右心房和左心室中NE、GAP-43、TH的含量降低(P<0.05);免疫荧光示各药物组右心房和左心室中TH阳性神经分布少于模型对照组(P<0.05)。桂芍比为1∶1和1∶2时药效最佳,与美托洛尔相似。结论桂枝汤(桂芍比为1∶1和1∶2)可以有效减轻4-MC诱导的心脏交感神经芽生。Aim To investigate the preventive effect of Guizhi decoction on cardiac sympathetic sprouting induced by 4-Methylcatechol （4-MC） . Methods The rat models of cardiac sympathetic sprouting were in-duced by 10 mg · L-1 4-MC （ 10μg · kg-1 body weight, i. p. ） . Guizhi decoctions （ with different pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba, 1 ： 1, 1 ： 2 and 2 ： 1） and metoprolol were administered to the rats. Heart rate and electrocardio-gram （ ECG ） were observed, the content of norepi-nephrine （NE）, growth associated protein （GAP-43）, tyrosine hydroxylase （ TH ） and acetylcholine transfer-ase enzyme （ CHAT） in myocardial homogenate of left ventricular and right atrial were determined by ELISA method, and immunofluorescence assay was used to observe cardiac nerve sprouting and sympathetic distri-bution. Results 4-MC caused cardiac sympathetic sprouting and parasympathetic was not influenced. Heart rate of the model group was improved significant-ly and higher than that of the other groups . Compared with the model group, the content of NE, GAP-43 and TH in left ventricle and right atrium of the metoprolol group and Guizhi decoction group were decreased（ P〈0.05 ） , and the immunofluorescence result showed that the distribution of TH positive nerve was reduced sig-nificantly（P〈0.05）. It was demonstrated that Guizhi decoction of the proportion between Ramulus Cinnamo-mi and Radix Paeoniae Alba 1 ： 1 and 1 ： 2 had the best efficacy, which was similar to the efficacy of meto-prolol. Conclusion Guizhi decoction （ with the pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba 1 ： 1 and 1 ： 2 ） effectively inhibits the cardi-ac sympathetic sprouting induced by 4-MC.

关 键 词：交感神经芽生 自主神经重构 4-甲基邻苯二酚 美托洛尔 桂枝汤 心脏 

分 类 号：R-332[医药卫生] R282.71