GLP-1受体激动剂艾塞那肽对肿瘤细胞增殖、迁移的影响  

GLP-1 agonist exenatide inhibits migration and proliferation in tumor cells

作　　者：张瑞[1] 刘羞菲[1] 王慧[1] 王小翠[1] 童强[1] 郑宏庭[1] 

机构地区：[1]第三军医大学新桥医院内分泌科,重庆400037

出　　处：《第三军医大学学报》2014年第17期1785-1789,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81270893);国家自然科学基金海外及港澳学者合作研究基金(81228023);重庆市自然科学基金重点项目(CSTC2012jjB10023);第三军医大学留学回国人员科研启动基金(2011XHG07);重庆市留学人员科技项目择优资助(渝留助20120003);教育部留学归国科研启动基金(2013A387)~~

摘　　要：目的目的通过体外细胞实验观察胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)受体激动剂艾塞那肽对乳腺癌、结肠癌、胰腺癌3种肿瘤细胞增殖、迁移的影响。方法选取乳腺癌MDA-MB-231细胞、结肠癌HCT116细胞、胰腺癌HS766T细胞作为研究对象,每种细胞分为对照组、二甲双胍组、艾塞那肽组。利用CCK-8分别检测3种肿瘤细胞艾塞那肽干预72 h后的增殖能力;利用Transwell小室法观察培养12 h后3种肿瘤细胞的迁移能力。结果艾塞那肽组与对照组相比,MDA-MB-231细胞、HCT116细胞的增殖受到明显抑制(P<0.01,P<0.05),而HS766T细胞增殖无影响(P>0.05)。Transwell体外细胞迁移实验结果显示,艾塞那肽组与对照组相比,MDA-MB-231细胞、HCT116细胞迁移受到明显抑制(P<0.01,P<0.05),但HS766T细胞迁移却显著增加(P<0.01)。结论 GLP-1受体激动剂艾塞那肽可以抑制乳腺癌、结肠癌细胞的增殖与迁移,不影响胰腺癌细胞增殖,却促进其迁移,其对不同肿瘤细胞的影响具有差异性。Objective To determine the effect of glucagon-like peptide-1 （ GLP-1 ） agonist exenatide on the proliferation and migration in breast cancer, colon cancer, and pancreatic cancer cells in vitro. Methods Breast cancer cell line MDA-MB-231, colon cancer cell line HCT116 and pancreatic cell line HS766T were selected as experimental subjects. Each type of cells was divided into control group, mefformin group and exenatide group. After administration of mefformin and exenatide respectively, CCK-8 assay was used for the proliferation detection in each group in 72 h after treatment, and the migration of tumor cells was observed using Transwell chamber assay in 12 h after treatment. Results Compared with the control group, the proliferation of MDA-MB-231 cells in exenatide group was significantly inhibited （P 〈 0.01 ） , the proliferation of HCT116 ceils were inhibited （ P 〈 0.05 ）, and the proliferation of HS766T was not affected （ P 〉 0.05 ）. Transwell migration assay showed that MDA-MB-231 cell migration was significantly inhibited by exenatide （P 〈 0.01 ） , HCT116 cell migration was also inhibited （ P 〈 0.05 ）, but cell migration in HS766T group was significantly increased by exenatide （P 〈 0.01 ）. Oonclusion Exenatide inhibits the proliferation and migration in breast cancer ceils and colon cancer cells. It has no effect on the proliferation, but promotes the migration in pancreatic cancer cells. Exenatide exerts different effects on different tumor cells.

关 键 词：艾塞那肽 乳腺肿瘤 结肠肿瘤 胰腺肿瘤 肿瘤细胞 培养的 

分 类 号：R73-37[医药卫生—肿瘤] R730.23[医药卫生—临床医学]