恒河猴慢性高眼压模型视乳头和视神经纤维损伤模式  

Patterns of optic nerve head and retinal nerve fiber layer damage in the monkey chronicocular hypertension model

作　　者：涂姝[1] 李康[1] 黄晶晶[1] 葛坚[1] 

机构地区：[1]眼科学国家重点实验室中山大学中山眼科中心,广州510060

出　　处：《中华眼视光学与视觉科学杂志》2014年第7期436-440,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：基金项目：国家自然科学基金（81170846,81371007）

摘　　要：目的观察恒河猴慢性高眼压模型与开角型青光眼患者的视乳头和视神经纤维损伤模式是否一致。方法实验研究。采用眼科多波长激光仪光凝恒河猴（12眼）的小梁网以破坏小梁网功能使眼压升高，建立恒河猴慢性高眼压模型。术前和术后每周行眼压、眼底照相、视乳头参数和视神经纤维层厚度检测。结果建模的12眼术前眼压为（16．08±2．02）mmHg，中央角膜厚度为（489．17±17．82）μm，眼轴为（20．32±0．84）mm。12只用于诱导建模的恒河猴眼，经3～4次激光光凝，11眼被成功诱导。27周平均眼压为（30．32±14．59）mmHg，眼压波动幅度为（8．19±7．45）mmHg。光学相关断层扫描成像显示整个盘沿面积从（1．67±0．44）mm2减小到（0．43±0．34）mm2。平均视神经纤维从（97．92±6．79）μm减少到（64．46±17．44）μm。结论恒河猴慢性高眼压性模型模拟了人类高眼压／开角型青光眼发病机制和过程，在视杯对高眼压的反应、视杯可逆性缩小、视神经纤维损伤现象等方面一致，是开展高眼压性视乳头和视神经纤维层损伤研究理想的动物模型。Objective To investigate if the chronic ocular hypertension （COHT） in a monkey model presents indistinguishable from humans in its retinal nerve fiber layer （RNFL） and optic nerve head （ONH） damage. Methods This was an experimental study. Models of COHT were induced in 12 eyes of rhesus monkeys were induced by using VISULAS Trion to disturb the aqueous humor outflow and cause the elevation of intraocular pressure. Some measurements have been taken such as intraocular pressure （IOP）, fundus photography, the ONH parameters, and the RNFL thickness were taken every week before and after laser treatment. Results The main results were as follows： the IOP of the twelve eyes of the monkeys was 16.08±2.02 mmHg, the central corneal thickness was 489.17±17.82 μm and the ocular axials length was 20.32±0.84 mm. Eleven eyes, underwent 3 to 4 laser photocoagulation treatments, were induced to the COHT model. The average IOP in 27 weeks was 30.32±14.59 mmHg, and the variation was 8.19±7.45 mmHg. Optical coherence tomography displayed revealed the a lost rim area that changed from 1.67±0.44 mm2 to 0.43±0.34 mm2 in the monkey COHT model. The RNFL was changed from 97.92±6.79 μm to 64.46±17.44 μm. Conclusion The monkey COHT model mimicks the natural ocular hypertension/open angle glaucoma procedure and shares similar glaucomatous damage patterns, such as the reactivity of ONH to ocular hypertension, and the presence of the optic cup and the damage phenomenon of RNFL. The monkey model is an ideal animal model for studying damage of the optic nerve head and RNFL.

关 键 词：恒河猴 动物模型 高眼压 视神经纤维 

分 类 号：R775[医药卫生—眼科]